PMID: 7543351Apr 1, 1995Paper

Development of an efficient serum-free semisolid culture system for the evaluation of hematopoietic progenitors

Journal of Hematotherapy
I VianesJ Reiffers

Abstract

Three different combinations of serum-free (SF) media proposed by Drouet et al., Ieki et al., and Wu et al. were tested to assess their ability to replace fetal calf serum (FCS) in a human hematopoietic progenitor semisolid culture system using human bone marrow and peripheral blood stem cells. This study confirmed that two (Drouet and Wu) of the three SF media tested were able to induce progenitor growth in the presence of either 5637-conditioned medium or a defined combination of growth factors: stem cell factor (SCF), interleukin-1 (IL-1), interleukin-3 (IL-3), and erythropoietin (EPO). The best results were obtained using the SF media described by Wu. The number of 5637-stimulated CFU-GM obtained with the Wu SF media was not different from that obtained with FCS-supplemented media when cultures were assessed on day 14 (79 +/- 14 versus 104 +/- 17), day 18 (64 +/- 11 versus 79 +/- 12), or day 21 (58 +/- 13 versus 62 +/- 12). Similar findings were obtained when the previously defined combination of growth factors was used to stimulate progenitor growth. Because the Wu medium was the most efficient SF medium to promote hematopoietic progenitor growth, we attempted to improve its efficacy by modifying the concentrations of the ...Continue Reading

Citations

Apr 5, 1994·Biotechnology and Bioengineering·C E SandstromE T Papoutsakis
Feb 13, 2004·The Journal of Trauma·Adena J OsbandDavid H Livingston
Dec 1, 1996·Journal of Hematotherapy·I D LewisL B To

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