PMID: 11334962May 4, 2001Paper

Development of an ELISA to measure soluble CD163 in biological fluids

Journal of Immunological Methods
Timothy H SulahianPaul M Guyre

Abstract

CD163 is a monocyte/macrophage restricted transmembrane glycoprotein and a member of the scavenger receptor cysteine-rich (SRCR) family of proteins. SRCR proteins are typically associated with the immune system. The regulation of CD163 by cytokines and glucocorticoids suggests that it plays a role in inflammatory processes. While CD163 is expressed as a membrane-bound protein, it has been shown to be actively shed from the surface of monocytes in a protease-dependent fashion when cells are stimulated with a phorbol ester. To better elucidate the function and biological importance of CD163, we have developed a solid-phase sandwich enzyme linked immunosorbant assay (ELISA) for the detection of soluble CD163 in biological fluids. This assay has good repeatability both within and between runs (coefficients of variation (CVs) of 3.2% and 7.1% or better, respectively). While detection of CD163 was inhibited by ethylenediamine tetraacetic acid (EDTA), CD163 immunoreactivity was not altered by the addition of heparin or hemoglobin. This report details the development of this novel assay for soluble CD163 and provides the first evidence of CD163 immunoreactivity in normal plasma and serum samples.

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Citations

Dec 30, 2004·Cytometry. Part B, Clinical Cytometry·Bruce H Davis, Petar V Zarev
Nov 2, 2001·Biochemical and Biophysical Research Communications·P Högger, C Sorg
Dec 2, 2010·Rheumatology International·Wakana NakayamaHironobu Ihn
Feb 21, 2002·The International Journal of Biochemistry & Cell Biology·Jonas Heilskov GraversenSøren K Moestrup
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Aug 18, 2006·Critical Care Medicine·Holger Jon MøllerGitte Kronborg
Jun 30, 2011·Indian Journal of Plastic Surgery : Official Publication of the Association of Plastic Surgeons of India·Andrzej PiatkowskiNorbert Pallua
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Jul 29, 2005·Pathology, Research and Practice·Atsushi HiraokaMorikazu Onji
Nov 9, 2011·Scandinavian Journal of Clinical and Laboratory Investigation·Holger J Møller
Sep 3, 2010·Journal of Leukocyte Biology·Anders EtzerodtSøren Kragh Moestrup
Apr 13, 2004·Journal of Leukocyte Biology·Timothy H SulahianPaul M Guyre
Dec 14, 2006·Journal of Leukocyte Biology·Lehn K WeaverPaul M Guyre
Sep 5, 2014·Experimental and Therapeutic Medicine·Shufang YuanYuexin Zhang
Feb 7, 2003·Scandinavian Journal of Clinical and Laboratory Investigation. Supplementum·H J MøllerS K Moestrup
May 14, 2020·Cells·Marlene Christina NielsenHenning Grønbæk

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