Development of an enzyme assay for rapid assessment of Escherichia coli in seawaters

Journal of Applied Microbiology
G CarusoM Mancuso

Abstract

An analytical protocol has been developed and applied for the detection of glucuronidase activity in marine waters as a rapid alternative approach to assess the microbiological quality of seawaters. The fluorogenic substrate 4-methylumbelliferyl-beta-D-glucuronide is cleaved to a fluorescent product, methylumbelliferone, by the enzyme beta-glucuronidase, specific to Escherichia coli and closely related enterobacterial species (Shigella). The results suggest that this test is related to E. coli numbers, as estimated by immunofluorescence, more significantly than to faecal coliform numbers, obtained from culture media. The determination of the potential rate of glucuronidase activity may be used as a diagnostic tool for the indirect estimation of the presence of E. coli in seawaters. The method may be particularly useful in the early warning of seawater pollution, allowing the screening of coastal areas with different contamination levels in reduced time.

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Citations

Jun 7, 2005·Marine Pollution Bulletin·Philippe LebaronP Servais
Jan 19, 2016·Enzyme and Microbial Technology·Nikou HesariMorteza Abbaszadegan
Mar 9, 2005·Letters in Applied Microbiology·T Garcia-ArmisenP Servais
May 20, 2008·Current Opinion in Biotechnology·Liv Fiksdal, Ingun Tryland
Feb 16, 2010·Water Research·Dirk WildeboerRamadan A Abuknesha
Nov 9, 2019·Analytical and Bioanalytical Chemistry·Ciprian Briciu-BurghinaFiona Regan

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