Development of an enzyme-linked immunosorbent assay to determine puerarin and its aglycone daidzein.

Journal of Natural Medicines
Benyakarn PongkitwitoonWaraporn Putalun

Abstract

Pueraria candollei (White Kwao Khuer) is a medicinal plant containing puerarin (8-C-glucosyl-4',7-dihydroxyisoflavone) and its aglycone, daidzein, as major isoflavonoids used for its rejuvenating and estrogenic effects. In order to analyze these isoflavonoids, an enzyme-linked immunosorbent assay (ELISA) was developed using polyclonal antibody (PAb) against puerarin. Puerarin was conjugated with bovine serum albumin (BSA) to be used as an immunogen for production of PAb from a rabbit. The PAb has been shown to be specific to puerarin and its aglycone, daidzein. An ELISA was then performed using anti-puerarin PAb in the range of 6.10 ng/ml-1.56 μg/ml of puerarin. Based on validation analysis, this analytical method by ELISA is a precise, accurate, and sensitive method for determining puerarin and daidzein in plant samples.

References

Jun 28, 2003·Journal of Natural Products·Feng-Lin HsuJuei-Tang Cheng
Jun 10, 2004·Journal of Agricultural and Food Chemistry·Jeevan K PrasainStephen Barnes
Nov 4, 2004·Journal of Pharmaceutical and Biomedical Analysis·Qunlin ZhangHua Cui
Jul 13, 2005·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Zhongze MaScott E Lukas
Jan 24, 2006·The Journal of Nutritional Biochemistry·Shuihua ZhangJianwen Liu
May 3, 2006·Biological & Pharmaceutical Bulletin·Sanyin ZhangHongxi Xu
Sep 25, 2007·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·W Cherdshewasart, W Sutjit
Aug 5, 2008·Cell Biology International·Hai-Ying ZhangHai-Tao Hu
Apr 4, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Suqin Han
Dec 5, 2009·Zeitschrift Für Naturforschung. C, a Journal of Biosciences·Latiporn UdomsukWaraporn Putalun

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Citations

Mar 4, 2014·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Huihua QuQingguo Wang
Apr 22, 2015·The American Journal of Tropical Medicine and Hygiene·John P RenschlerRamanan Laxminarayan
Jan 22, 2014·The American Journal of Tropical Medicine and Hygiene·Carla Cristina Squaiella-BaptistãoDenise V Tambourgi

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