Development of an Enzyme-Linked Immunosorbent Assay To Detect Antibodies Targeting Recombinant Envelope Protein 2 of Mayaro Virus

Journal of Clinical Microbiology
Marcílio Jorge FumagalliLuiz Tadeu Moraes Figueiredo

Abstract

Mayaro virus (MAYV) is a neglected arthropod-borne virus (arbovirus) antigenically clustered into the Semliki Forest complex group of Alphavirus genus (Togaviridae family), maintained in an unclear zoonotic cycle involving mosquitoes from Haemagogus genus as the main vector. The genome is composed of a positive single-stranded RNA of 11.5 kb in length, which contains two genes that encode four nonstructural (nsP1 to nsP4) and five structural (C, E3, E2, 6K, and E1) proteins. In the present study, we have developed an enzyme-linked immunosorbent assay (ELISA) using as antigen the recombinant envelope protein 2 of MAYV produced in an Escherichia coli system (rE2-MAYV ELISAs). A panel of 68 human serum samples from suspected arboviral cases was analyzed and titrated for anti-MAYV IgM and IgG antibody detection. The rE2-MAYV ELISA detected 33.8% (23/68) IgG-positive samples, demonstrating 100% sensitivity and 78.95% specificity compared to the MAYV-specific 50% plaque reduction neutralization assay. In addition, the positive MAYV-neutralizing samples showed high titers of detection by rE2-MAYV ELISA, suggesting a highly sensitive test. The rE2-MAYV ELISA also detected 42.5% (29/68) IgM-positive samples, of which 13.8% (4/29) presen...Continue Reading

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Citations

Apr 26, 2020·Transactions of the Royal Society of Tropical Medicine and Hygiene·Marilia Farignoli RomeiroLuiz Tadeu Moraes Figueiredo
Sep 4, 2019·PLoS Pathogens·Luiza A de Castro-JorgeDario S Zamboni
Jul 2, 2020·Tropical Medicine and Infectious Disease·Carla Julia da Silva Pessoa VieiraGabriel da Luz Wallau
Jul 30, 2021·Scientific Reports·Paloma Napoleão-PêgoSalvatore G De-Simone
Sep 23, 2021·Journal of Virology·Marcilio Jorge FumagalliLuiz Tadeu Moraes Figueiredo

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