PMID: 8601712Mar 28, 1996Paper

Development of an enzyme-linked immunosorbent assay for human plasma inter-alpha-trypsin inhibitor (ITI) using specific antibodies against each of the H1 and H2 heavy chains

Journal of Immunological Methods
C MizonJ Mizon

Abstract

Inter-alpha-trypsin inhibitor (ITI) is a serine-proteinase inhibitor of human plasma enzymes. ITI is composed of three polypeptide chains covalently linked: bikunin, responsible for the antiprotease activity and two heavy chains H1 and H2. Human plasma also contains other components immunologically related to ITI such as pre-alpha-trypsin inhibitor (paI), inter-alpha-like inhibitor (IalphaLI) and free bikunin. The ELISA procedure we propose exclusively measures native ITI within the range 12.5-200 microgram/l. The intra- and interassay coefficients of variation were less than 5.6% and 8.7%, respectively. When ITI was added to plasma samples, full recovery was obtained. EDTA-plasma from 30 healthy individuals revealed a mean level of 241.5 mg/l (range 145.5-506). The high specificity, sensitivity, reproducibility and accuracy of the present assay should facilitate the specific measurement of native ITI in blood and thus might represent a useful tool for further physiopathological studies.

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Citations

Feb 19, 1998·British Journal of Urology·C J DawsonR L Ryall
Sep 10, 2011·Obstetrics and Gynecology International·Hiromitsu YabushitaAkihiko Wakatsuki
Dec 31, 1997·American Journal of Respiratory and Critical Care Medicine·M JourdainC Chopin
Sep 5, 2002·Clinical Chemistry and Laboratory Medicine : CCLM·Charlotte MizonJacques Mizon
Oct 28, 1997·The Journal of Laboratory and Clinical Medicine·D AlbaniJ Mizon
Jan 19, 2018·International Journal of Molecular Sciences·Eva Nagyova
Sep 21, 2012·Kansenshōgaku zasshi. The Journal of the Japanese Association for Infectious Diseases·Akiko MaedaYoshio Hirota
Sep 30, 2016·The Journal of Biological Chemistry·Aaron C Petrey, Carol A de la Motte
Mar 1, 2000·The Journal of Laboratory and Clinical Medicine·M BalduyckJ Mizon

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