Development of an enzyme-linked immunosorbent assay for atrazine mercapturic acid in human urine

Chemical Research in Toxicology
L L JaegerB D Hammock

Abstract

Improved assessments of human exposure to electrophilic chemicals require rapid and inexpensive analytical techniques that can detect specific urinary metabolites at low levels as needed for epidemiological screenings of large populations. The first aim of this study has been to apply rational hapten design strategies to develop a more sensitive and selective enzyme-linked immunosorbent assay for atrazine mercapturic acid. Polyclonal sheep antiserum was generated against an improved hapten, numerous coating antigen chemistries were evaluated, and assay conditions were optimized. An assay was developed with an IC50 of 0.08 +/- 0.02 micrograms/L (K approximately with 10(-)10 M) for atrazine mercapturic acid. The assay exhibited greatest recognition of atrazine mercapturic acid relative to other known urinary metabolites of atrazine as well as other triazine herbicides. The assay was surprisingly selective to atrazine mercapturic acid over the structurally similar simazine mercapturic acid. Urine samples presented matrix effects due in part to the nonspecific effects of urinary salts, but 4-fold dilution of urine achieved an overall method limit of quantitation of 0.3 micrograms/L. Solid-phase extraction strategies were also devel...Continue Reading

References

Jan 1, 1992·Critical Reviews in Toxicology·E Nelson
Jan 1, 1990·Bulletin of Environmental Contamination and Toxicology·G CatenacciL Pozzoli
Jan 1, 1989·Critical Reviews in Toxicology·R F HendersonJ D Sun
Jan 1, 1994·International Archives of Occupational and Environmental Health·H Inagaki, M Minami
Aug 1, 1993·Toxicology Letters·G CatenacciM Maroni

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Citations

Jan 18, 2006·Analytical and Bioanalytical Chemistry·Ki Chang AhnBruce D Hammock
Mar 27, 2012·Analytical and Bioanalytical Chemistry·Nuria TortM-Pilar Marco
May 11, 2005·International Archives of Occupational and Environmental Health·V Haufroid, D Lison
Oct 1, 2003·Biotechnology Advances·Kerrm Y F YauJ Christopher Hall
Jun 28, 2011·Arhiv za higijenu rada i toksikologiju·Gordana Mendaš
Feb 9, 2010·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·Mohan SinghRavi Kiran
Aug 17, 2000·The Journal of Laboratory and Clinical Medicine·A V LyubimovS E Baker
Sep 9, 2017·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·Arthur D ZimmermanChad D Foradori
Apr 10, 2007·Therapeutic Drug Monitoring·Maria G MargaritiAristidis M Tsatsakis
Aug 2, 2005·Mass Spectrometry Reviews·Karen BrownIan N H White

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