Development of an ex vivo technique to achieve reanimation of hearts sourced from a porcine donation after circulatory death model

The Journal of Surgical Research
Omar A MownahDavid Talbot

Abstract

This study reports on the development of a novel method for achieving ex vivo reanimation of hearts from a porcine donation after circulatory death (DCD) model without the use of donor pretreatment. Porcine hearts (n = 23) were procured 10-29 min after confirmation of asystole. All hearts underwent initial flush with AQIX RS-I solution (London, UK). A 2-h preservation period followed: group 1 hearts (n1-n11) were preserved using static cold storage, group 2 hearts (n12-n17) were preserved using oxygenated, hypothermic machine perfusion (MP), and group 3 hearts (n18-n23) were subjected to retrograde oxygen persufflation. Reperfusion was performed on a Langendorff modification of a Model 33 Functional Circulation circuit. In hearts n16-n23, a dialysis circuit was incorporated into the circuit to facilitate removal of metabolites. The experimental protocol was allowed to follow an evolutionary course, with the aim of achieving greater success with reanimation. In group 1 (static cold storage), 7 of the 11 hearts (63.6%) achieved reanimation on the ex vivo circuit. Two of the six hearts (33.3%) in group 2 (MP) were successfully reanimated. All the six hearts (100%) in group 3 (persufflation) were successfully reanimated. The period...Continue Reading

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Citations

Mar 6, 2015·European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery·Herman TolboomVolkmar Falk
Apr 6, 2017·Current Opinion in Organ Transplantation·Kumud K DhitalPeter S Macdonald
Apr 11, 2018·Current Opinion in Organ Transplantation·Catherine G Min, Klearchos K Papas
Apr 24, 2018·Current Opinion in Organ Transplantation·Alyssa WardJedediah K Lewis

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