Development of an experimental apparatus and protocol for determining antimicrobial activities of gaseous plant essential oils

International Journal of Food Microbiology
Hyun-Sun SeoJee-Hoon Ryu

Abstract

There is a growing interest in the use of naturally-occurring antimicrobial agents such as plant essential oils (EOs) to inhibit the growth of hazardous and spoilage microorganisms in foods. Gaseous EOs (EO gases) have many potential applications in the food industry, including use as antimicrobial agents in food packaging materials and sanitizing agents for foods and food-contact surfaces, and in food processing environments. Despite the potentially beneficial applications of EO gases, there is no standard method to evaluate their antimicrobial activities. Thus, the present study was aimed at developing an experimental apparatus and protocol to determine the minimal inhibitory concentration (MIC) and minimal lethal concentration (MLC) of EO gases against microorganisms. A sealed experimental apparatus was constructed for simultaneous evaluation of antimicrobial activities of EO gases at different concentrations without creating concentration gradients. A differential medium was then evaluated in which a color change allowed for the determination of growth of glucose-fermenting microorganisms. Lastly, an experimental protocol for the assessment of MIC and MLC values of EO gases was developed, and these values were determined fo...Continue Reading

References

Feb 3, 1999·Regulatory Toxicology and Pharmacology : RTP·J N SofosE A Johnson
Jul 13, 2004·International Journal of Food Microbiology·Sara Burt
May 16, 2006·Nihon Ishinkin Gakkai zasshi = Japanese journal of medical mycology·Shigeharu InouyeShigeru Abe
Sep 21, 2010·International Journal of Food Microbiology·A K Tyagi, A Malik
Nov 22, 2011·International Journal of Food Microbiology·Raúl Avila-SosaAurelio López-Malo
Jan 8, 2013·International Journal of Food Microbiology·Hyejung JunJee-Hoon Ryu

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Citations

Mar 19, 2019·Critical Reviews in Food Science and Nutrition·Fatima Reyes-JuradoRaúl Ávila-Sosa
May 19, 2020·Journal of the Science of Food and Agriculture·Laura Navarro-SeguraAntonio López-Gómez

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