Development of an Image-Guided Orthotopic Xenograft Mouse Model of Endometrial Cancer with Controllable Estrogen Exposure

International Journal of Molecular Sciences
Gonda Fj KoningsAndrea Romano

Abstract

Endometrial cancer (EC) is the most common gynaecological malignancy in Western society and the majority of cases are estrogen dependent. While endocrine drugs proved to be of insufficient therapeutic value in the past, recent clinical research shows promising results by using combinational regimens and pre-clinical studies and identified potential novel endocrine targets. Relevant pre-clinical models can accelerate research in this area. In the present study we describe an orthotopic and estrogen dependent xenograft mouse model of EC. Tumours were induced in one uterine horn of female athymic nude mice using the well-differentiated human endometrial adenocarcinoma Ishikawa cell line-modified to express the luciferase gene for bioluminescence imaging (BLI). BLI and contrast-enhanced computed-tomograph (CE-CT) were used to measure non-invasive tumour growth. Controlled estrogen exposure was achieved by the use of MedRod implants releasing 1.5 μg/d of 17β-estradiol (E2) in ovariectomized mice. Stable E2 serum concentration was demonstrated by LC-MS/MS. Induced tumours were E2 responsive as increased tumour growth was observed in the presence of E2 but not placebo, assessed by BLI, CE-CT, and tumour weight at sacrifice. Metastatic...Continue Reading

References

Apr 20, 2007·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·S B Decruze, J A Green
Dec 6, 2011·General and Comparative Endocrinology·E IngbergJ O Strom
Dec 27, 2011·Clinical & Experimental Metastasis·Silvia CabreraJaume Reventós
Mar 1, 2012·The Journal of Clinical Endocrinology and Metabolism·Karlijn M C CornelAndrea Romano
Aug 17, 2012·The Cochrane Database of Systematic Reviews·Claire L ValePaul R Symonds
Aug 30, 2012·Nature Methods·Caroline A SchneiderKevin W Eliceiri
Dec 24, 2014·European Journal of Cancer Prevention : the Official Journal of the European Cancer Prevention Organisation (ECP)·Salvatore GizzoGiovanni Battista Nardelli
Jan 28, 2015·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Brian M SlomovitzRobert L Coleman
Aug 8, 2015·PloS One·Ingfrid S HaldorsenCamilla Krakstad
Sep 12, 2015·Lancet·Philippe MoriceEmile Darai
Mar 1, 2016·Frontiers in Pharmacology·Tea Lanišnik Rižner
Mar 11, 2016·Journal of Veterinary Science & Technology·Laurivette MosqueraAnnabell C Segarra
Jul 8, 2016·Oncotarget·Ingvild Løberg TangenCamilla Krakstad
Jan 28, 2017·BJOG : an International Journal of Obstetrics and Gynaecology·A E DerbyshireE J Crosbie
Jul 12, 2017·Frontiers in Pharmacology·Maša SinreihTea Lanišnik Rižner
Feb 8, 2018·Journal of Pharmaceutical and Biomedical Analysis·Merja R HäkkinenSeppo Auriola

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Methods Mentioned

BETA
xenografts
xenograft
genetic modification
sedation
X-ray
biopsies

Clinical Trials Mentioned

NCT01797523
NCT03008408
NCT02730923
NCT02228681
NCT02657928
NCT03077698
NCT02730429

Software Mentioned

KaleidaGraph
PMOD
ImageJ

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