PMID: 11902914Mar 21, 2002Paper

Development of an immunoassay for the residues of the herbicide bensulfuron-methyl

Journal of Agricultural and Food Chemistry
Jae Koo LeeDae-Whang Kim

Abstract

To develop a competitive indirect enzyme-linked immunosorbent assay based on polyclonal antibodies for the detection of the sulfonylurea herbicide bensulfuron-methyl, seven structurally related haptens were synthesized. Four of them mimicking the target analyte were conjugated to keyhole limpet hemocyanin by the N-hydroxysuccinimide activated ester method to use as immunogens, and all of them were conjugated to bovine serum albumin to use as plate-coating antigens. Polyclonal antibodies raised in rabbits and the coating antigens were screened and selected for the assay in simple homologous and heterologous ELISA formats. Three sensitive heterologous ELISAs were selected and optimized, showing the average IC(50) values of bensulfuron-methyl as low as 0.17, 0.09, and 0.09 ng/mL, the detection ranges of 0.04-0.60, 0.01-0.60, and 0.04-0.25 ng/mL, and the lowest detection limits of 0.03, 0.002, and 0.03 ng/mL, respectively. The cross-reactivities of other sulfonylurea herbicides and metabolites of bensulfuron-methyl to the antibodies were less than 15% in the two assays. Recoveries from the analyte-fortified water samples in assay I were in the range of 81-125% by simple dilution. The correlation between the ELISA and HPLC was 0.999...Continue Reading

References

Jan 11, 1980·Journal of Chromatography·P HartvigO Gyllenhaal
Dec 20, 1999·Journal of Agricultural and Food Chemistry·G ShanB D Hammock

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Citations

Jan 18, 2006·Analytical and Bioanalytical Chemistry·Ki Chang AhnBruce D Hammock
Oct 1, 2003·Biotechnology Advances·Kerrm Y F YauJ Christopher Hall
Nov 20, 2009·Environmental Science & Technology·Ki Chang AhnBruce D Hammock
Jun 19, 2013·Journal of Agricultural and Food Chemistry·Anupama RanganathanBruce D Hammock
Jan 1, 2008·Acta Crystallographica. Section E, Structure Reports Online·Jin-Yun XiaDeng-Yu Wu
Aug 1, 2013·World Journal of Microbiology & Biotechnology·Zhen Hong ZhuangShi Hua Wang
Mar 5, 2016·Environmental Science & Technology·Ki Chang AhnShirley J Gee
Aug 10, 2015·Analytical and Bioanalytical Chemistry·Anupama RanganathanBruce D Hammock
Aug 20, 2020·Organic & Biomolecular Chemistry·Alexey V KletskovAntonio Frontera
Aug 5, 2010·Journal of Agricultural and Food Chemistry·Celia Suárez-PantaleónAntonio Abad-Fuentes
Aug 12, 2008·Journal of Agricultural and Food Chemistry·Josep V MercaderAntonio Abad-Fuentes

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