Development of an impedimetric immunosensor based on electropolymerized polytyramine films for the direct detection of Salmonella typhimurium in pure cultures of type strains and inoculated real samples

Analytica Chimica Acta
Antonios V PournarasMamas I Prodromidis

Abstract

The development of a faradic impedimetric immunosensor based on electropolymerized polytyramine (Ptyr) films for the detection of S. typhimurium in milk is described for the first time. Polyclonal anti-Salmonella was cross-linked, in the presence of glutaraldehyde vapors, on Ptyr-modified gold electrodes. The dielectric behaviour of Ptyr films was evaluated with capacitance measurements, while their stability in neutral aqueous solutions was examined with impedimetric measurements. The effect of the concentration of tyramine in the forming solution on both the sensitivity and the dynamic range of the resulted immunosensors was also investigated. The alteration of the interfacial features of the electrodes due to different modification or recognition steps, was measured by faradic electrochemical impedance spectroscopy in the presence of a hexacyanoferrate(II)/(III) redox couple. At samples containing a low initial concentration of 10 cfu mL(-1)S. typhimurium, that actually defines the LOD of the immunosensors, signal changes of 33% and 88% were achieved after 3 and 10 h incubation, respectively. To achieve the working simplicity expected by a biosensor, immunoreaction was performed directly in cultures. This resulted in the eli...Continue Reading

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Citations

May 19, 2010·Analytical Chemistry·Benjamin J PrivettMark H Schoenfisch
Sep 12, 2014·Artificial Cells, Nanomedicine, and Biotechnology·Elif Burcu Bahadır, Mustafa Kemal Sezgintürk
Mar 6, 2012·Biosensors & Bioelectronics·Timothy R J HolfordSéamus P J Higson
Dec 18, 2010·Biosensors & Bioelectronics·Yi WanBaorong Hou
Jun 2, 2009·Biosensors & Bioelectronics·Vanessa Escamilla-GómezJosé M Pingarrón
May 4, 2016·Applied Microbiology and Biotechnology·Airis Maria Araújo MeloCarlúcio R Alves
Jul 2, 2014·Clinical Microbiology Reviews·Asif AhmedPaul A Millner
Apr 5, 2020·Medical Microbiology and Immunology·J Leva-BuenoP A Millner
Jun 26, 2020·Food Science and Biotechnology·Changhoon Chai, Se-Wook Oh
May 5, 2020·Biosensors & Bioelectronics·Ellen Cesewski, Blake N Johnson

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