Development of an implantable D-serine biosensor for in vivo monitoring using mammalian D-amino acid oxidase on a poly (o-phenylenediamine) and Nafion-modified platinum-iridium disk electrode

Biosensors & Bioelectronics
Zainiharyati M ZainSulaiman Ab Ghani

Abstract

D-serine has been implicated as a brain messenger, promoting not only neuronal signalling but also synaptic plasticity. Thus, a sensitive tool for D-serine monitoring in brain is required to understand the mechanisms of D-serine release from glia cells. A biosensor for direct fixed potential amperometric monitoring of D-serine incorporating mammalian D-amino acid oxidase (DAAO) immobilized on a Nafion coated poly-ortho-phenylenediamine (PPD) modified Pt-Ir disk electrode was therefore developed. The combined layers of PPD and Nafion enhanced the enzyme activity and biosensor efficiency by approximately 2-fold compared with each individual layer. A steady state response time (t(90%)) of 0.7+/-0.1s (n=8) and limit of detection 20+/-1 nM (n=8) were obtained. Cylindrical geometry showed lower sensitivity compared to disk geometry (61+/-7 microA cm(-2) mM(-1), (n=4), R(2)=0.999). Interference by ascorbic acid (AA), the main interference species in the central nervous system and other neurochemical electroactive molecules was negligible. Implantation of the electrode and microinjection of D-serine into rat brain striatal extracellular fluid demonstrated that the electrode was capable of detecting D-serine in brain tissue in vivo.

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Citations

Aug 7, 2012·Amino Acids·Zainiharyati Mohd ZainRobert D O'Neill
Dec 4, 2012·Enzyme and Microbial Technology·Makoto YoshimotoRyoichi Kuboi
Mar 15, 2011·Trends in Biotechnology·Loredano Pollegioni, Gianluca Molla
Mar 7, 2018·Biosensors·Diana Campos-BeltránLisa Marshall
Jul 28, 2020·Sensors·Clara Pérez-RàfolsGastón A Crespo
Aug 14, 2021·International Journal of Biological Macromolecules·Sachchidanand Soaham GuptaKumar Rakesh Ranjan
Jan 5, 2011·The Journal of Physical Chemistry. B·Massimo Di FuscoFranco Mazzei
Mar 22, 2014·Analytical Chemistry·David PolcariJanine Mauzeroll

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