Development of an improved genotyping assay for the detection of hepatitis C virus genotypes and subtypes in Pakistan

Journal of Virological Methods
Muhammad Idrees

Abstract

A new genotyping system was established for the specific detection of HCV genotypes 1a, 1b, 1c, 2a, 2b, 2c, 3a, 3b, 3c, 4a-h, 5a and 6a during the course of this study. The system is based on entire core region and a part of 5' noncoding region (5'NCR) with genotype-specific primers. Genotype-specific primers were designed on the basis of 114 HCV isolates. Serum samples with known genotypes were used as positive controls to validate the assay developed and to generate PCR band patterns. Band patterns generated from the clinical serum samples from HCV patients were compared to the patterns produced from these control samples. In addition, the type-specific bands were sequenced from the test patients and control clinical samples to validate further the test results. To determine sensitivity and specificity of the assay, a total 260 samples were analyzed simultaneously by this HCV genotyping method and that developed by Ohno and Murex HCV Serotyping 1-6 Assay. The system showed 79.2% concordance with Ohno's system and 65.38% with serotyping system. Samples with discordant results were sequenced and their genotypes were determined by molecular evolutionary analysis. The data indicate that the method described in this study may offe...Continue Reading

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Citations

Jan 7, 2011·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·S ButtI Ur Rehman
Apr 10, 2013·Asian Pacific Journal of Tropical Biomedicine·Sher Zaman SafiMuhammad Ashraf
Jun 30, 2011·Virology Journal·Latif ur RehmanAnila Tariq Jahangiri
Aug 2, 2011·Emerging Infectious Diseases·Sadia ButtIram Amin
Oct 28, 2009·World Journal of Gastroenterology : WJG·Muhammad IdreesSheikh Riazuddin
Sep 8, 2010·World Journal of Gastroenterology : WJG·Waqar AhmadSajida Hassan
Aug 8, 2013·Hepatitis Monthly·Keivan Majidzadeh-AHossain Jabbari
Dec 22, 2010·Expert Review of Molecular Diagnostics·Reem R Al Olaby, Hassan M E Azzazy
May 5, 2010·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Sadia ButtShazia Rafique
Aug 1, 2009·Journal of Virological Methods·Cintia CostiMaria Lucia Rosa Rossetti
Dec 25, 2008·Journal of Medical Virology·Victoria L DemetriouLeondios G Kostrikis
May 28, 2011·Journal of Digestive Diseases·Muhammad IdreesAbrar Hussain
May 11, 2011·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Haji AkbarSana Saleem
Dec 28, 2010·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Muhammad IdreesFayyaz Ahmed Malik
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