Development of an in vitro blood-brain barrier model to study the effects of endosulfan on the permeability of tight junctions and a comparative study of the cytotoxic effects of endosulfan on rat and human glial and neuronal cell cultures

Environmental Toxicology
Melissa P L ChanMinoru Yoneda

Abstract

Endosulfan, an organochlorine (OC) insecticide that belongs to the cyclodiene group, is one of the most commonly used pesticides to control pests in vegetables, cotton, and fruits. Porcine brain microvascular endothelial cells were used to develop a model to study the effects of endosulfan on the permeability of tight junctions in the blood-brain barrier (BBB). BBB permeability, measured as transendothelial electrical resistance, decreased in a dose- and time-dependent manner when treated with alpha-endosulfan, beta-endosulfan, or endosulfan sulfate. Cytotoxicity testing revealed that the three endosulfans did not cause cell death at concentrations of 10 microM and below. The ratio of the average permeability of the filter-grown endothelial cell monolayer to 14C-endosulfan (Pe) going from the outer to the inner compartments with that going from the inner to the outer compartments was approximately 1:1.2-2.1 after exposure to concentrations of 0.01-10 microM. alpha-Endosulfan, beta-endosulfan, and endosulfan sulfate had cytotoxic effects on rat glial (C6) and neuronal (PC12) cell cultures as well as on human glial (CCF-STTG1) and neuronal (NT2) cell cultures. The effects of alpha-endosulfan were highly selective, with a wide ran...Continue Reading

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Citations

Feb 24, 2019·Toxicological Sciences : an Official Journal of the Society of Toxicology·Chunjuan SongAnumantha G Kanthasamy
Nov 28, 2019·Environmental Toxicology and Chemistry·Rodrigo Hérnan Da CuñaKarina Silvia Beatriz Miglioranza
Aug 4, 2015·Regenerative Medicine·Kyle D FinkJan A Nolta
Jun 15, 2019·Acta Neuropathologica·Jason R RichardsonAnumantha G Kanthasamy
Aug 21, 2020·Environmental Science and Pollution Research International·Ashif IqubalSyed Ehtaishamul Haque
Jul 23, 2021·Advanced Science·Elise P W JenkinsGeorge G Malliaras

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