Development of an in vitro colony formation assay for the evaluation of in vivo chemotherapy of a rat brain tumor.

In Vitro
M L RosenblumC B Wilson

Abstract

An in vitro colony formation assay for the evaluation of in vivo brain tumor therapy has been developed. When plated, disaggregated cells derived from solid tumors proliferated to form relatively homogeneous colonies after a latency period of 2 to 6 days. Increasing concentrations of fetal calf serum enhanced colony-forming efficiency (CFE) with a plateau between 7 and 16%. Supplementation with either irradiated feeder cells (10(3) to 10(5) cells per dish), or medium conditioned by 1 to 3 days of in vitro incubation with the same cell line, doubled the CFE. The density of tumor cells (untreated or previously treated with chemotherapeutic agents) did not affect the CFE when a minimum of 10(4) total cells (tumor plus feeder) were plated. Therefore, in this system the optimal experimental conditions for evaluating chemotherapy and radiotherapy require incubation of disaggregated tumor cells for 12 days in medium containing 10% of fetal calf serum and enough feeder cells to provide a minimum of 10(4) cells per dish. The CFE for untreated tumors was 18 +/- 10% (+/-S.D.), demonstrating that there is significant biological variation. The assay appeared sensitive, with reproducible results, when applied to individual chemically treated...Continue Reading

References

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Citations

Jan 1, 1981·Journal of Neurology·M WeizsaeckerM Barker
Jan 1, 1994·Cancer Chemotherapy and Pharmacology·N RoosenM L Rosenblum
Nov 26, 1999·International Journal of Radiation Oncology, Biology, Physics·J H KimS L Brown
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Sep 12, 2006·Radiation and Environmental Biophysics·Yang JiansheWalsh Linda
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