Development of an in vitro model of essential fatty acid deficiency in fish cells

Prostaglandins, Leukotrienes, and Essential Fatty Acids
D R TocherJ R Sargent

Abstract

Levels of eicosapentaenoic acid (EPA; 20:5, n-3) greatly exceed those of arachidonic acid (AA; 20:4, n-6) in the tissue phospholipids of most fish species. Despite this, it is 20:4, n-6-derived eicosanoids that are produced predominantly in fish cells. The development of an essential fatty acid (EFA)-deficient fish cell line would greatly assist the study of this selectivity and so several fish cell lines were cultured in EFA-deficient (EFAD) media. All n-3 and n-6 polyunsaturated fatty acids (PUFA) and total PUFA were considerably reduced in all lines, except turbot fin (TF) in which total n-9 PUFA doubled from 13.8% to 27.5% of total fatty acids. In the topminnow hepatocarcinoma cell line (PLHC-1), there was almost complete depletion of both n-3 and n-6 PUFA and in TF cells, no n-3 PUFA were detected. In the carp epithelial papilloma cell line (EPC), both n-6 and n-3 PUFA were reduced by approximately 70%. The reduced PUFA in cells cultured in EFAD media was compensated to a large extent in most cell lines by significantly increased percentages of monounsaturated fatty acids, particularly 18:1, n-9. Total n-9 PUFA were significantly increased in all cell lines by culture in EFAD media, with 20:2, n-9 significantly increased i...Continue Reading

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Citations

Jun 9, 2016·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Diana B ReisCovadonga Rodríguez
Nov 3, 2010·Health & Social Care in the Community·Julie McGarryKathryn Hinchliff-Smith
Jan 1, 1996·Archiv für Tierernährung·A G Tacon
Nov 26, 2019·Bioscience, Biotechnology, and Biochemistry·Akifumi ShiomiMasato Umeda

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