Development of an in vitro photosensitization test based on changes of cell-surface thiols and amines as biomarkers: the photo-SH/NH₂ test

The Journal of Toxicological Sciences
Shiho OedaHirokazu Kouzuki

Abstract

As a part of our studies to develop a cell-based in vitro photosensitization assay, we examined whether changes of cell-surface thiols and amines on human monocytic cell line THP-1 could be used to predict photosensitizing potential of chemicals. First, we identified a suitable ultraviolet A (UV-A) irradiation dose to be 5.0 J/cm(2) by investigating the effect of UV-A on the levels of cell-surface thiols and amines in ketoprofen (KP; a representative photoallergen)-treated THP-1 cells. Next, we confirmed that phenol red, a known photoirritant used as a pH indicator in the culture medium, did not affect the KP-induced changes of cell-surface thiols and amines. Using the criterion of more than 15% change of cell-surface thiols and/or amines in response to UV-A irradiation, 22 of 26 known photosensitizers (15 of 18 photoallergens, 7 of 8 photoirritants) were judged positive. Seven of 7 known non-phototoxins did not alter cell-surface thiols or amines. The accuracy for predicting photosensitizers was 87.9% (sensitivity/specificity; 84.6%/100%), and the accuracy for predicting photoallergens was 69.7% (sensitivity/specificity; 83.3%/53.3%). Our results suggest that changes of cell-surface thiols and/or amines may be useful biomarker...Continue Reading

References

Nov 25, 2000·Alternatives to Laboratory Animals : ATLA·W W Lovell, P A Jones
Nov 9, 2002·Toxicology in Vitro : an International Journal Published in Association with BIBRA·T AshikagaS Aiba
Nov 9, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Giuseppe FilomeniMaria Rosa Ciriolo
Jan 28, 2004·American Journal of Contact Dermatitis : Official Journal of the American Contact Dermatitis Society·Maeran LandersFrances J Storrs
Jul 16, 2004·Toxicological Sciences : an Official Journal of the Society of Toxicology·G Frank GerberickJean-Pierre Lepoittevin
Oct 12, 2004·Photochemistry and Photobiology·Johnson J InbarajColin F Chignell
Jun 15, 2006·Journal of Applied Toxicology : JAT·Ian R JowseyIan Kimber
Dec 5, 2006·Journal of Dermatological Science·Masako KuritaYoshiki Tokura
Feb 1, 2008·Toxicology and Applied Pharmacology·Joan E RobertsColin F Chignell
Aug 12, 2008·Journal of Dermatological Science·Ryosuke HinoYoshiki Tokura
Apr 11, 2009·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Masatoshi HoyaSetsuya Aiba
May 16, 2009·Journal of Photochemistry and Photobiology. B, Biology·Brian HenryKaren Alsante
Jun 6, 2009·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Mie SuzukiSetsuya Aiba
Dec 25, 2010·Toxicology in Vitro : an International Journal Published in Association with BIBRA·V GalbiatiE Corsini
Nov 15, 2012·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Yuko NukadaNaohiro Nishiyama
Mar 6, 2013·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Morihiko HirotaSetsuya Aiba
May 15, 2013·Journal of Applied Toxicology : JAT·Joanna JaworskaAndreas Natsch
Jul 3, 2013·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Valentina GalbiatiEmanuela Corsini
Oct 19, 2013·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Satomi OnoueShizuo Yamada
Jan 5, 2014·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Satomi OnoueHajime Kojima
Jan 22, 2014·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Kyoko Tsujita-InoueSetsuya Aiba

❮ Previous
Next ❯

Citations

Apr 6, 2018·The Journal of Toxicological Sciences·Akemi Toyoda, Hiroshi Itagaki
Jan 5, 2021·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Satomi OnoueHiroshi Onodera

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Toxicology in Vitro : an International Journal Published in Association with BIBRA
Masatoshi HoyaSetsuya Aiba
Toxicology in Vitro : an International Journal Published in Association with BIBRA
Mie SuzukiSetsuya Aiba
Toxicology in Vitro : an International Journal Published in Association with BIBRA
Valentina GalbiatiEmanuela Corsini
Toxicology in Vitro : an International Journal Published in Association with BIBRA
Verónica MartínezMontserrat Mitjans
Journal of Applied Toxicology : JAT
Kyoko Tsujita-InoueHirokazu Kouzuki
© 2022 Meta ULC. All rights reserved