Development of an in vitro tumor spheroid culture model amenable to high-throughput testing of potential anticancer nanotherapeutics

Journal of Liposome Research
Melani A SolomonGerard G M D'Souza

Abstract

Three-dimensional tumor spheroid cultures are a better representative of in vivo solid tumors than monolayer cultures and should be used for testing potential nanotherapeutics in vitro. To develop techniques to test the disposition and efficacy of nanocarrier formulations in spheroids in a cost-effective manner amenable to high-throughput testing. Spheroids were obtained using a modified liquid overlay technique in a 96-well plate. Several nanocarrier formulations were prepared and tested in the spheroid model. The disposition of the formulations in the spheroids was determined by confocal microscopy while the effect of the drug-loaded formulations was assessed in terms of the cell viability, loss of membrane integrity, induction of caspases and inhibition of growth of the spheroids. The surface charge of the formulations influenced the accumulation of the nanocarrier and drug in the spheroid, with the cationic formulation accumulating to the greatest extent. Also, the smallest particle size formulation, micelles, penetrated to the greatest extent in the spheroid. The iRGD tumor-penetrating peptide co-administered with unmodified liposomes exhibited both high accumulation and penetration. The effect studies revealed that the fo...Continue Reading

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Citations

Jan 13, 2018·Frontiers in Oncology·Frédérique MittlerMaxim Y Balakirev
Aug 9, 2017·Biotechnology Journal·Chiara MoriconiMark Gumbleton
Nov 22, 2018·Journal of Drug Targeting·Giovanna LolloJean-Pierre Benoit
Dec 10, 2020·Pharmaceutics·Bárbara PintoHassan Bousbaa
Nov 15, 2020·Nanomaterials·Indra Van ZundertSusana Rocha
Apr 11, 2021·Advanced Drug Delivery Reviews·Sabina PozziRonit Satchi-Fainaro

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