Development of an In Vivo and In Vitro Ileal Fermentation Method in a Growing Pig Model

The Journal of Nutrition
Carlos A MontoyaP J Moughan

Abstract

Substantial microbial fermentation may occur mainly in the lower small intestine (SI) of human adults, but there is no established methodology to determine this. The study aimed to develop a combined in vivo and in vitro methodology for ileal fermentation based on the pig as an animal model for digestion in human adults. Several aspects of a combined in vivo/in vitro ileal fermentation assay were evaluated. Male 9-wk-old pigs (n = 30; mean ± SD body weight: 23 ± 1.6 kg) were fed a human-type diet (143, 508, 45, 49, and 116 g/kg dry matter diet of crude protein, starch, total lipid, ash, and total dietary fiber) for 15 d. On day 15, pigs were killed, and the last third of the SI was collected to prepare an ileal digesta-based inoculum. Terminal jejunal digesta (last 50 cm of the second third of the SI) were collected as substrate for the assay to test the form of substrate (fresh or freeze-dried), origin (location in jejunum or SI) of the substrate, storage of the inoculum, incubation time (1.2-6.8 h), pH of the medium, and inoculum concentration (6-26 mg inoculum/100 mg substrate). The group of donor pigs used to prepare the inoculum, form of the substrate, origin of the substrate, origin of the inoculum (location in the SI), s...Continue Reading

References

Jan 1, 1992·World Review of Nutrition and Dietetics·P J MoughanM Pedraza
Jun 1, 1991·The Journal of Applied Bacteriology·J H Cummings, G T Macfarlane
Jan 1, 1987·Scandinavian Journal of Gastroenterology. Supplement·H Graham, P Aman
Feb 1, 1989·Journal of Medical Microbiology·M DanH J Koornhof
Nov 1, 1985·The American Journal of Clinical Nutrition·H N Englyst, J H Cummings
Jul 1, 1986·The American Journal of Clinical Nutrition·H N Englyst, J H Cummings
May 1, 1987·The American Journal of Clinical Nutrition·J H Cummings, H N Englyst
Jan 1, 1987·Annual Review of Nutrition·E R Miller, D E Ullrey
Mar 1, 1970·The British Journal of Nutrition·S E Nixon, G E Mawer
Feb 1, 1983·The American Journal of Clinical Nutrition·W D HollowayK Maher
Feb 1, 1984·The American Journal of Clinical Nutrition·N I McNeil
Jan 19, 2000·European Journal of Nuclear Medicine·R BenninkL Mortelmans
Apr 25, 2001·Clinical Physiology·J GraffJ L Madsen
Jun 24, 2003·Cryobiology·Zdenek Hubálek
Oct 1, 1957·The Journal of Clinical Investigation·B BORGSTROMJ SJOVALL
Jan 13, 2006·Proceedings of the National Academy of Sciences of the United States of America·Elisabeth M BikDavid A Relman
Apr 15, 2008·Experimental Biology and Medicine·Jannine K PattersonDennis D Miller
Feb 23, 2012·Scientific Reports·Jennifer C StearnsJosh D Neufeld
Jul 11, 2012·Journal of the Science of Food and Agriculture·Dongjie WangBruce R D'Arcy
Oct 31, 2012·The British Journal of Nutrition·Amelie Deglaire, Paul J Moughan
Feb 20, 2013·Journal of the Science of Food and Agriculture·Leah T ColesAlison J Darragh
Oct 19, 2013·Nutrition Research Reviews·Sonja N HeinritzEva Weiss
Sep 10, 2014·Journal of Microbiological Methods·Marisol AguirreKoen Venema
Jul 30, 2015·Journal of Microbiological Methods·Marisol AguirreKoen Venema

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