Development of an in vivo glucosylation platform by coupling production to growth: Production of phenolic glucosides by a glycosyltransferase of Vitis vinifera

Biotechnology and Bioengineering
Frederik De BruynMarjan De Mey

Abstract

Glycosylation of small molecules can significantly alter their properties such as solubility, stability, and/or bioactivity, making glycosides attractive and highly demanded compounds. Consequently, many biotechnological glycosylation approaches have been developed, with enzymatic synthesis and whole-cell biocatalysis as the most prominent techniques. However, most processes still suffer from low yields, production rates and inefficient UDP-sugar formation. To this end, a novel metabolic engineering strategy is presented for the in vivo glucosylation of small molecules in Escherichia coli W. This strategy focuses on the introduction of an alternative sucrose metabolism using sucrose phosphorylase for the direct and efficient generation of glucose 1-phosphate as precursor for UDP-glucose formation and fructose, which serves as a carbon source for growth. By targeted gene deletions, a split metabolism is created whereby glucose 1-phosphate is rerouted from the glycolysis to product formation (i.e., glucosylation). Further, the production pathway was enhanced by increasing and preserving the intracellular UDP-glucose pool. Expression of a versatile glucosyltransferase from Vitis vinifera (VvGT2) enabled the strain to efficiently p...Continue Reading

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Citations

Dec 15, 2015·Biotechnology Advances·Katharina SchmölzerBernd Nidetzky
Aug 22, 2015·Applied Microbiology and Biotechnology·Motomitsu Kitaoka
Oct 13, 2016·Journal of Agricultural and Food Chemistry·Jianjun PeiYongde Yue
May 31, 2019·Applied Microbiology and Biotechnology·Karel OlavarriaSebastian Aljoscha Wahl
Nov 12, 2017·Applied Microbiology and Biotechnology·Jia WangYajun Yan
Mar 27, 2019·Engineering in Life Sciences·Renkuan ZhangYing-Jin Yuan
May 22, 2021·Journal of Agricultural and Food Chemistry·Tian LiuHuifeng Jiang
Oct 27, 2021·Journal of Agricultural and Food Chemistry·Yaping MaoYong Wang

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