PMID: 11312816Apr 21, 2001Paper

Development of an indirect competitive ELISA for ciprofloxacin residues in food animal edible tissues

Journal of Agricultural and Food Chemistry
J Duan, Z Yuan

Abstract

An indirect competitive enzyme-linked immunosorbent assay (ELISA) was developed to detect ciprofloxacin (CPFX) in food animal edible tissues. CPFX was converted by an active ester method into conjugates CPFX-bovine serum albumin (CPFX-BSA) and CPFX-human serum albumin (CPFX-HSA), which both allowed production of CPFX-specific rabbit antisera. In the ELISA, CPFX-HSA was coated onto the microtiter plate, followed by incubation with standard CPFX and anti-CPFX antibody. The indirect competitive ELISA revealed that the antisera have no cross-reactivity with penicillin, gentamicin, neomycin, sulfadiazine, and chlortetracycline. The antisera cross-reacted with enrofloxacin and norfloxacin about 69.8 and 44.6% as much as they did with CPFX. This ELISA was highly sensitive (0.32 ng/mL) to CPFX determination. Recovery of CPFX at 40 microg/kg was 75.58% in pork, 81.29% in chicken, and 84.30% in milk. The coefficients of variation varied from 3.7 to 9.2% over the range of CPFX concentrations studied. The linear detection range was between 1.6 and 1000 ng/mL. The results suggest that this ELISA is a specific, accurate, and convenient method for the detection of CPFX residues in food animal edible tissues.

References

Aug 14, 1999·Journal of Veterinary Pharmacology and Therapeutics·H García OvandoI Puelles

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Citations

Mar 3, 2007·Analytical and Bioanalytical Chemistry·Limin CaoVladimir M Mirsky
Mar 14, 2013·Journal of Agricultural and Food Chemistry·Pan HuShiying Lu
Aug 11, 2011·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·D PengZ Yuan
Apr 29, 2015·Journal of the Science of Food and Agriculture·Jiahong ChenHongtao Lei
May 30, 2015·Journal of Immunoassay & Immunochemistry·Yinli Zhao, Guoxi Li
Apr 13, 2010·Journal of Immunological Methods·Francisco Bruno Moreira Brás GomesJoão Paulo Medeiros Ferreira
Aug 16, 2014·Journal of Hazardous Materials·Rong ZhangLi-Ping Yu
Apr 20, 2016·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Mi-Sun HaDong-Ho Bae
May 31, 2013·Journal of the South African Veterinary Association·Ajay K OlaBibhuti Ranjan
Jan 20, 2006·Journal of Veterinary Pharmacology and Therapeutics·R GehringJ E Riviere
Mar 20, 2010·Journal of Enzyme Inhibition and Medicinal Chemistry·Prabodh Chander SharmaMohammad Shahar Yar
Apr 11, 2014·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Mi-Sun HaWoon-Seok Yeo
Jan 12, 2013·Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes·Yan Z LiuJian P Wang
Dec 5, 2008·Journal of Agricultural and Food Chemistry·Yinli ZhaoJianhua Wang
Aug 20, 2008·Journal of Agricultural and Food Chemistry·Jin-E WuDong-Ping He
Apr 1, 2008·Journal of Agricultural and Food Chemistry·Josep V MercaderAntonio Abad-Fuentes
Jan 6, 2009·Journal of Agricultural and Food Chemistry·Sijun ZhaoJianzhong Shen
Jun 16, 2009·Journal of Agricultural and Food Chemistry·Wei ShengTing Xu
Aug 5, 2004·Journal of Agricultural and Food Chemistry·Els Van CoillieWim Reybroeck
Feb 12, 2008·Journal of Agricultural and Food Chemistry·Chao ChangZong-hui Yuan

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