Development of an integrated microfluidic solid-phase extraction and electrophoresis device

The Analyst
Suresh KumarAdam T Woolley

Abstract

This study focuses on the design and fabrication of a microfluidic platform that integrates solid-phase extraction (SPE) and microchip electrophoresis (μCE) on a single device. The integrated chip is a multi-layer structure consisting of polydimethylsiloxane valves with a peristaltic pump, and a porous polymer monolith in a thermoplastic layer. The valves and pump are fabricated using soft lithography to enable pressure-based fluid actuation. A porous polymer monolith column is synthesized in the SPE unit using UV photopolymerization of a mixture consisting of monomer, cross-linker, photoinitiator, and porogens. The hydrophobic, porous structure of the monolith allows protein retention with good through flow. The functionality of the integrated device in terms of pressure-controlled flow, protein retention and elution, on-chip enrichment, and separation is evaluated using ferritin (Fer). Fluorescently labeled Fer is enriched ∼80-fold on a reversed-phase monolith from an initial concentration of 100 nM. A five-valve peristaltic pump produces higher flow rates and a narrower Fer elution peak than a three-valve pump operated under similar conditions. Moreover, the preconcentration capability of the SPE unit is demonstrated through...Continue Reading

References

Feb 24, 2001·Journal of Chromatography. a·S Ulrich
Sep 28, 2002·Science·Todd ThorsenStephen R Quake
Feb 5, 2003·Current Opinion in Biotechnology·Brian M PaegelRichard A Mathies
Mar 12, 2003·Advanced Drug Delivery Reviews·Bernhard H WeiglCatherine R Cabrera
Sep 2, 2003·Analytical Chemistry·Janusz Pawliszyn
Mar 25, 2004·Journal of Mass Spectrometry : JMS·György Vas, Károly Vékey
Dec 30, 2004·Analytical Chemistry·Robert S FooteJ Michael Ramsey
Mar 25, 2005·BioTechniques·Gina S Fiorini, Daniel T Chiu
Jul 28, 2006·Nature·George M Whitesides
Dec 22, 2009·Integrative Biology : Quantitative Biosciences From Nano to Macro·Jungkyu KimBruce K Gale
Nov 11, 2011·Journal of Chromatography. a·Jörg P Kutter
Sep 22, 2012·Journal of Chromatography. a·Pamela N NgeAdam T Woolley
Feb 16, 2013·Chemical Reviews·Pamela N NgeAdam T Woolley
Jul 3, 2013·Journal of Separation Science·Kun LiuMilton L Lee
Nov 7, 2014·Analytical Chemistry·Frantisek Svec, Yongqin Lv
Jun 21, 2015·Analytica Chimica Acta·Eduardo Carasek, Josias Merib
Nov 6, 2015·Analytical and Bioanalytical Chemistry·V SahoreA T Woolley

❮ Previous
Next ❯

Citations

May 18, 2016·Biomicrofluidics·Radim KnobAdam T Woolley
May 5, 2017·The Analyst·Mohamed DawodRobert T Kennedy
May 13, 2017·Analytical Methods : Advancing Methods and Applications·Mukul SonkerAdam T Woolley
Apr 24, 2018·Electrophoresis·Anna V NielsenAdam T Woolley
Oct 21, 2016·Analytical and Bioanalytical Chemistry·Colleen E DuganRobert T Kennedy
Aug 10, 2017·Electrophoresis·María Ramos-PayánMiguel Ángel Bello-López
Nov 3, 2016·Journal of Separation Science·Sille Štěpánová, Václav Kašička

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.