Development of an on-line control system for the cultivation of animal cells in a hollow-fiber reactor using flowing injection analysis and a visual programming language

Journal of Biotechnology
T S StollI W Marison

Abstract

A system for the independent control of ammonia and glutamine in a hollow-fiber reactor has been developed. On-line analysis of the two chemical species was performed using FIA. A custom-made program has been written to fully automate the FIA, to perform measurements, calibrations and data acquisition, and to activate two peristaltic feed pumps. Diagnostic tests were also automatically performed in order to identify, and in some cases eliminate, erroneous measurements and calibrations due to instrument failures. For this purpose, a visual programming language (LabVIEW) has been chosen. Ammonia concentration was controlled by varying the medium supply rate to the hollow-fiber reactor which was shown to behave like an ideal-stirred tank reactor. The glutamine concentration was controlled through the intermittent activation of a pump delivering a concentrated solution of this species to the reactor. This control system was tested with a culture of hybridoma cells at three different ammonia and one glutamine set-points. The hollow-fiber reactor was successfully operated automatically for more than 1200 h with ammonia and glutamine concentrations accurately controlled at non-limiting levels. The visual programming environment was fo...Continue Reading

References

Jun 1, 1991·Journal of Biotechnology·B SonnleitnerA Fiechter
Jan 1, 1995·Critical Reviews in Biotechnology·A Mulchandani, A S Bassi
Apr 15, 1994·Journal of Biotechnology·M E GregoryN F Thornhill
Dec 15, 1995·Journal of Biotechnology·J J van der PolC Wandrey
Feb 1, 1995·Cytotechnology·T StollI W Marison

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Citations

Apr 1, 1997·Current Opinion in Biotechnology·A RitzkaT Scheper
Mar 29, 2014·PloS One·David A Burdge, Igor G L Libourel
Nov 18, 2009·Biotechnology Progress·Gayle E DerfusAshraf Amanullah
Jan 1, 2010·Biotechnology Progress·Maria Elisa RodriguesRosário Oliveira
Apr 1, 1999·Biotechnology and Bioengineering·J E DowdK E Kwok

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