Development of an Optimized Protocol for NMR Metabolomics Studies of Human Colon Cancer Cell Lines and First Insight from Testing of the Protocol Using DNA G-Quadruplex Ligands as Novel Anti-Cancer Drugs

Metabolites
Ilaria LauriAntonio Randazzo

Abstract

The study of cell lines by nuclear magnetic resonance (NMR) spectroscopy metabolomics represents a powerful tool to understand how the local metabolism and biochemical pathways are influenced by external or internal stimuli. In particular, the use of adherent mammalian cells is emerging in the metabolomics field in order to understand the molecular mechanism of disease progression or, for example, the cellular response to drug treatments. Hereto metabolomics investigations for this kind of cells have generally been limited to mass spectrometry studies. This study proposes an optimized protocol for the analysis of the endo-metabolome of human colon cancer cells (HCT116) by NMR. The protocol includes experimental conditions such as washing, quenching and extraction. In order to test the proposed protocol, it was applied to an exploratory study of cancer cells with and without treatment by anti-cancer drugs, such as DNA G-quadruplex binders and Adriamycin (a traditional anti-cancer drug). The exploratory NMR metabolomics analysis resulted in NMR assignment of all endo-metabolites that could be detected and provided preliminary insights about the biological behavior of the drugs tested.

References

Jul 16, 1981·The New England Journal of Medicine·R C YoungC E Myers
Apr 20, 2001·Proceedings of the National Academy of Sciences of the United States of America·M ReadS Neidle
Aug 1, 1959·Canadian Journal of Biochemistry and Physiology·E G BLIGH, W J DYER
Sep 29, 2004·Journal of Cellular Physiology·Uwe WenzelHannelore Daniel
Nov 3, 2004·Journal of Experimental Botany·P KrishnanR G Ratcliffe
Feb 4, 2005·Toxicological Sciences : an Official Journal of the Society of Toxicology·Donald G Robertson
Apr 21, 2006·Nature·T Andrew ClaytonJeremy K Nicholson
Oct 13, 2007·The Journal of Clinical Investigation·Erica SalvatiAnnamaria Biroccio
May 12, 2009·Nucleic Acids Research·Jianguo XiaDavid S Wishart
Jun 12, 2009·Current Opinion in Chemical Biology·Shankar Balasubramanian, Stephen Neidle
Dec 17, 2009·Journal of Magnetic Resonance·F SavoraniS B Engelsen
Jul 6, 2010·Drug Discovery Today·Miroslava Cuperlović-CulfIan Chute
Jul 31, 2010·The FEBS Journal·Tracy A BrooksLaurence Hurley
Feb 8, 2011·CA: a Cancer Journal for Clinicians·Ahmedin JemalDavid Forman
Aug 13, 2011·Analytical and Bioanalytical Chemistry·Estelle MartineauSerge Akoka
Sep 29, 2011·Metabolomics : Official Journal of the Metabolomic Society·Jing BaiWei Ning Chen
Nov 9, 2011·Cell Research·Athanasia D PanopoulosJuan Carlos Izpisua Belmonte
Mar 2, 2012·Current Genomics·Jane Tang
Oct 13, 2012·Journal of Medicinal Chemistry·Sandro CosconatiAntonio Randazzo
Nov 20, 2012·Nucleic Acids Research·David S WishartAugustin Scalbert
Feb 21, 2013·Nature Chemistry·Giulia BiffiShankar Balasubramanian
May 2, 2013·Journal of Proteome Research·Neil J LoftusAlan J Barnes
Aug 31, 2013·Omics : a Journal of Integrative Biology·Aihua ZhangXijun Wang
Sep 17, 2013·Metabolomics : Official Journal of the Metabolomic Society·Bei CaoJiye Aa
Oct 11, 2013·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Joanna BidzinskaMarco Folini
Nov 22, 2013·Journal of Medicinal Chemistry·Francesco Saverio Di LevaAnnamaria Biroccio
Oct 17, 2014·Journal of Chemical Information and Modeling·Obaid AftabMats G Gustafsson
Nov 11, 2014·Metabolomics : Official Journal of the Metabolomic Society·Xuan WangJian Zhi Hu

❮ Previous
Next ❯

Methods Mentioned

BETA
NMR
scraping
PCA

Software Mentioned

Matlab
MetaboAnalyst
Chenomx NMR Suite
Simca
Statistical Total Correlation Spectroscopy ( STOCSY )
Chenomx NMR

Related Concepts

Related Feeds

Cancer Metabolism

In order for cancer cells to maintain rapid, uncontrolled cell proliferation, they must acquire a source of energy. Cancer cells acquire metabolic energy from their surrounding environment and utilize the host cell nutrients to do so. Here is the latest research on cancer metabolism.

Cancer Metabolism: Therapeutic Targets

Targeting the mechanisms by which cancer cells acquire energy for metabolic needs is a therapeutic target. Discover the latest research on cancer metabolism and therapeutic targets.

Cancer -Omics

A variety of different high-throughput technologies can be used to identify the complete catalog of changes that characterize the molecular profile of cohorts of tumor samples. Discover the latest insights gained from cancer 'omics' in this feed.

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

© 2022 Meta ULC. All rights reserved