Development of an UPLC-MS/MS method for simultaneous quantitation of 11 d-amino acids in different regions of rat brain: Application to a study on the associations of d-amino acid concentration changes and Alzheimer's disease

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Zhe LiYan Cui

Abstract

There are significant differences in d-amino acid concentrations between healthy people and Alzheimer's disease patients. In order to investigate the potential correlation between d-amino acids and Alzheimer's disease, a simple and sensitive ultra high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method has been developed. The method was applied to simultaneous determination of 11 d-amino acids in different regions of rat brain. Rat brain homogenates were firstly pretreated with protein precipitation procedure and then derivatized with (S)-N-(4-nitrophenoxycarbonyl) phenylalanine methoxyethyl ester [(S)-NIFE]. Baseline separation of the derivatives was achieved on an ACQUITY UPLC BEH C18 column (2.1 mm×50mm, 1.7μm). The mobile phase consisted of acetonitrile and water (containing 8mM ammonium hydrogen carbonate) and the flow rate was 0.6mLmin(-1). The derived analytes were sensitively detected by multiple reaction monitoring in the positive ion mode. The lower limits of quantitation ranged from 0.06 to 10ngmL(-1) with excellent linearity (r≥0.9909). The intra- and inter-day RSD were in the range of 3.6-12% and 5.7-12%, respectively. The recovery rate was 82.5%-95.3%. With this UPLC-MS/MS method, the 1...Continue Reading

Citations

Aug 29, 2020·Amino Acids·Weihua XuNing Zhang
Feb 23, 2019·Frontiers in Psychiatry·Mary-Anne B MacKayGlen B Baker
Mar 29, 2019·Frontiers in Molecular Biosciences·Danielle L GrahamDavid B Berkowitz
Sep 21, 2020·Biochimica Et Biophysica Acta. Proteins and Proteomics·E PlouxJ-M Billard
Nov 14, 2018·Journal of the American Society for Mass Spectrometry·Navid J AyonWilliam G Gutheil

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