Development of antigen-delivery systems, based on the Escherichia coli hemolysin secretion pathway

Gene
I GentschevW Goebel

Abstract

We describe the development of plasmid vectors carrying the expression sites, an hlyA cassette and the secretion genes of Escherichia coli hemolysin. These allow the synthesis and secretion of heterologous microbial antigens in E. coli and attenuated Salmonella aroA strains. Genes or gene fragments encoding microbial antigens are inserted in-frame into a residual part of the hlyA gene which essentially encodes the HlyA secretion signal (HlyAs). In general, the fused genes, carrying the hlyAs sequence at the 3' terminus, are efficiently expressed, and the synthesized antigens are secreted into the culture supernatant of the producing strain. Attenuated Salmonella strains synthesizing either HlyAs-fused listeriolysin or p60 of Listeria monocytogenes were constructed by this procedure and shown to provide protective immunity against L. monocytogenes in mice. The most effective protection was obtained when these microbial antigens were secreted by the attenuated Salmonella strains. We further present new approaches which may allow the application of this antigen-delivery system to any microbial antigen.

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Mar 1, 1992·Molecular & General Genetics : MGG·I Gentschev, W Goebel
Mar 1, 1991·Molecular Microbiology·R A Welch
Jun 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·C Wandersman, P Delepelaire
Nov 1, 1990·Molecular & General Genetics : MGG·J HessT Jarchau
Jun 1, 1988·Immunology Today·S H Kaufmann
Feb 1, 1995·Current Opinion in Cell Biology·P Cossart
Nov 1, 1994·Trends in Biotechnology·M A Blight, I B Holland
Aug 1, 1993·Current Opinion in Immunology·E G Pamer
Jan 18, 1994·Proceedings of the National Academy of Sciences of the United States of America·S J LibbyF Heffron
May 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·F ZhangV Ling
Feb 20, 1996·Proceedings of the National Academy of Sciences of the United States of America·J HessS H Kaufmann
Jun 1, 1996·Applied Microbiology and Biotechnology·H J MollenkopfW Goebel
Sep 13, 1996·Molecular & General Genetics : MGG·I GentschevW Goebel
Dec 1, 1996·Veterinary Microbiology·W C CrayH W Moon

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Citations

Sep 13, 1996·Molecular & General Genetics : MGG·I GentschevW Goebel
Jun 7, 2008·Applied Microbiology and Biotechnology·Lingyun ZhouYuanxing Zhang
Nov 17, 2007·Biotechnology Letters·Anisia J SilvaJorge A Benitez
Jul 15, 2009·Biotechnology Letters·Ye Ni, Rachel Chen
Oct 31, 2013·Biotechnology Letters·Alejandro Hochkoeppler
Jun 5, 2003·International Journal for Parasitology·Guido DietrichIvaylo Gentschev
Jun 7, 2003·Advances in Enzyme Regulation·Ulf R RappRudolf Götz
Mar 23, 2000·FEMS Immunology and Medical Microbiology·S SprengW Goebel
Mar 23, 2000·FEMS Immunology and Medical Microbiology·E Medina, C A Guzmán
Mar 23, 2000·FEMS Immunology and Medical Microbiology·D BumannT F Meyer
Jan 5, 2002·Trends in Microbiology·Ivaylo GentschevWerner Goebel
Oct 26, 2010·The Journal of Biological Chemistry·Patrick J BakkesLutz Schmitt
Mar 20, 2001·Infection and Immunity·S KöhlerJ P Liautard
Dec 8, 2007·Microbiology and Molecular Biology Reviews : MMBR·Joachim Jose, Thomas F Meyer
Jun 19, 2001·Biological Chemistry·H MollenkopfS H Kaufmann
Apr 21, 1999·Molecular Microbiology·D E HigginsD A Portnoy
Aug 13, 2011·Biotechnology Journal·Elisabeth LintonCharles D Miller
Dec 15, 2004·International Journal of Medical Microbiology : IJMM·Ivaylo GentschevUlf R Rapp
Feb 14, 2012·Human Vaccines & Immunotherapeutics·Donata R SizemoreKevin P Killeen
Jul 2, 2003·FEMS Immunology and Medical Microbiology·Yuanyi LiHeinz P Hahn
Jul 2, 2003·FEMS Immunology and Medical Microbiology·Heinz P Hahn, Bernd Ulrich von Specht
Sep 2, 2005·Vaccine·Donata R SizemoreKevin P Killeen
Apr 14, 2004·Critical Reviews in Clinical Laboratory Sciences·Sharmila ManojSylvia van Drunen Littel-van den Hurk

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