Development of Bacillus thuringiensis CryIC Resistance by Spodoptera exigua (Hubner) (Lepidoptera: Noctuidae).

Applied and Environmental Microbiology
W J MoarM J Adang

Abstract

Selection of resistance in Spodoptera exigua (Hubner) to an HD-1 spore-crystal mixture, CryIC (HD-133) inclusion bodies, and trypsinized toxin from Bacillus thuringiensis subsp. aizawai and B. thuringiensis subsp. entomocidus was attempted by using laboratory bioassays. No resistance to the HD-1 spore-crystal mixture could be achieved after 20 generations of selection. Significant levels of resistance (11-fold) to CryIC inclusion bodies expressed in Escherichia coli were observed after seven generations. Subsequent selection of the CryIC-resistant population with trypsinized CryIC toxin resulted, after 21 generations of CryIC selection, in a population of S. exigua that exhibited only 8% mortality at the highest toxin concentration tested (320 (mu)g/g), whereas the 50% lethal concentration was 4.30 (mu)g/g for the susceptible colony. Insects resistant to CryIC toxin from HD-133 also were resistant to trypsinized CryIA(b), CryIC from B. thuringiensis subsp. entomocidus, CryIE-CryIC fusion protein (G27), CryIH, and CryIIA. In vitro binding experiments with brush border membrane vesicles showed a twofold decrease in maximum CryIC binding, a fivefold difference in K(infd), and no difference in the concentration of binding sites for...Continue Reading

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Citations

May 14, 2005·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Joel S Griffitts, Raffi V Aroian
Nov 21, 2007·Applied and Environmental Microbiology·Konasale J AnilkumarWilliam J Moar
Dec 11, 2008·Annual Review of Entomology·Aaron J GassmannBruce E Tabashnik
Jul 21, 2009·Journal of Insect Science·Ann M ShowalterBrad Coates
Sep 20, 1997·The Journal of Biological Chemistry·B OppertW H McGaughey
Apr 16, 2013·Journal of Chemical Ecology·Dorothy J CheruiyotWilliam J Moar
Sep 30, 2000·Applied and Environmental Microbiology·B E TabashnikT J Dennehy
Mar 4, 2009·Pest Management Science·Patricia Hernández-MartínezBaltasar Escriche
Jul 8, 2015·Frontiers in Plant Science·Myriam SiegwartMiguel Lopez-Ferber
Sep 25, 2017·Pest Management Science·Hui TangYufang Peng
Mar 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·M KotaW J Moar
Dec 1, 2001·Annual Review of Entomology·Juan Ferré, Jeroen Van Rie
Mar 24, 2017·Journal of Economic Entomology·B PetersonJ Van den Berg
Mar 14, 2019·Archives of Insect Biochemistry and Physiology·Jizhen WeiShiheng An
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Jul 31, 2019·Current Opinion in Insect Science·Caroline Placidi de Bortoli, Juan Luis Jurat-Fuentes
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