Development of Biodegradable Polycation-Based Inhalable Dry Gene Powders by Spray Freeze Drying

Pharmaceutics
Tomoyuki OkudaHirokazu Okamoto

Abstract

In this study, two types of biodegradable polycation (PAsp(DET) homopolymer and PEG-PAsp(DET) copolymer) were applied as vectors for inhalable dry gene powders prepared by spray freeze drying (SFD). The prepared dry gene powders had spherical and porous structures with a 5~10-μm diameter, and the integrity of plasmid DNA could be maintained during powder production. Furthermore, it was clarified that PEG-PAsp(DET)-based dry gene powder could more sufficiently maintain both the physicochemical properties and in vitro gene transfection efficiencies of polyplexes reconstituted after powder production than PAsp(DET)-based dry gene powder. From an in vitro inhalation study using an Andersen cascade impactor, it was demonstrated that the addition of l-leucine could markedly improve the inhalation performance of dry powders prepared by SFD. Following pulmonary delivery to mice, both PAsp(DET)- and PEG-PAsp(DET)-based dry gene powders could achieve higher gene transfection efficiencies in the lungs compared with a chitosan-based dry gene powder previously reported by us.

References

Oct 2, 1998·Pharmaceutical Research·L Illum
Apr 1, 1999·Pharmaceutical Research·Y F MaaC C Hsu
Nov 21, 2000·Biotechnology and Bioengineering·M TservistasM Hoare
Jul 19, 2002·The Journal of Gene Medicine·P C SevilleJ C Birchall
Nov 16, 2002·Journal of Pharmaceutical Sciences·Taylor K C ArmstrongThomas J Anchordoquy
Jan 18, 2003·Journal of Pharmaceutical Sciences·Hirokazu OkamotoKazumi Danjo
Mar 26, 2003·Clinical and Experimental Immunology·S FerrariE Alton
Feb 11, 2004·Cellular and Molecular Life Sciences : CMLS·D R GillS C Hyde
Feb 25, 2005·Molecular Therapy : the Journal of the American Society of Gene Therapy·Abdelatif Elouahabi, Jean-Marie Ruysschaert
Jun 23, 2005·The Journal of Pharmacy and Pharmacology·Majella E LaneOwen I Corrigan
Jul 26, 2005·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Maytal Bivas-BenitaGerrit Borchard
Sep 2, 2005·Journal of Pharmaceutical Sciences·Nora Y K ChewHak-Kim Chan
Oct 18, 2005·Journal of Controlled Release : Official Journal of the Controlled Release Society·Naumana R Rabbani, Peter C Seville
Jan 25, 2007·Pharmaceutical Research·Albert H L ChowBoris Y Shekunov
Jun 7, 2007·Molecular Therapy : the Journal of the American Society of Gene Therapy·Keiji ItakaKazunori Kataoka
Jun 22, 2007·Journal of Controlled Release : Official Journal of the Controlled Release Society·Muri HanKazunori Kataoka
Aug 28, 2007·The Journal of Gene Medicine·Michael L EdelsteinJo Wixon
Sep 8, 2007·Journal of Pharmaceutical Sciences·Shigeru KawakamiMitsuru Hashida
Nov 28, 2007·Pharmaceutical Research·Reinhard Vehring
Dec 5, 2008·Chemical Reviews·Meredith A Mintzer, Eric E Simanek
Dec 23, 2008·Journal of Controlled Release : Official Journal of the Controlled Release Society·Tomoyuki MizunoHirokazu Okamoto
Apr 2, 2009·Molecular Therapy : the Journal of the American Society of Gene Therapy·Mariko Harada-ShibaKazunori Kataoka
Oct 15, 2009·International Journal of Pharmaceutics·Johannes GeigerCarsten Rudolph
Feb 27, 2010·Journal of Controlled Release : Official Journal of the Controlled Release Society·Kohta MohriHirokazu Okamoto
Jun 24, 2010·Macromolecular Bioscience·Lewis D HahnDavid J Mooney
Dec 28, 2010·Journal of Controlled Release : Official Journal of the Controlled Release Society·Hirokazu OkamotoYoshihiko Watanabe
Mar 8, 2011·Journal of Controlled Release : Official Journal of the Controlled Release Society·Takahiro NomotoKazunori Kataoka
May 24, 2011·Journal of Controlled Release : Official Journal of the Controlled Release Society·Corinna PfeiferCarsten Rudolph
Feb 16, 2012·Molecular Therapy : the Journal of the American Society of Gene Therapy·Satoshi UchidaKazunori Kataoka
May 25, 2013·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Wanling LiangJenny K W Lam
Apr 16, 2014·Advanced Drug Delivery Reviews·Qi Tony ZhouHak-Kim Chan
Apr 23, 2015·International Journal of Pharmaceutics·Stefan WanningAlf Lamprecht

❮ Previous
Next ❯

Citations

Jan 5, 2016·Biological & Pharmaceutical Bulletin·Yumiko SuzukiHirokazu Okamoto
Nov 3, 2017·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Tomoyuki Okuda
Dec 10, 2019·Expert Opinion on Drug Delivery·Nivedita ShettyQi Tony Zhou
Jun 9, 2020·Advanced Drug Delivery Reviews·Michael Y T ChowHak-Kim Chan
Jan 1, 2021·Pharmaceutics·Birendra Chaurasiya, You-Yang Zhao
Nov 13, 2020·Journal of Controlled Release : Official Journal of the Controlled Release Society·Ling DingDavid Oupický
Mar 12, 2021·Advanced Drug Delivery Reviews·Rachel Yoon Kyung ChangHak-Kim Chan
May 4, 2021·Chemical Reviews·Ramya KumarTheresa M Reineke
Aug 10, 2018·Molecular Pharmaceutics·Takaaki ItoHirokazu Okamoto

❮ Previous
Next ❯

Methods Mentioned

BETA
transfection
nuclear magnetic resonance
Electrophoresis
light scattering
Fluorescence
imaging technique

Software Mentioned

AEROSOL

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V
Wanling LiangJenny K W Lam
Journal of Controlled Release : Official Journal of the Controlled Release Society
Kohta MohriHirokazu Okamoto
Current Pharmaceutical Design
Michael Y T Chow, Jenny K W Lam
Journal of Controlled Release : Official Journal of the Controlled Release Society
V SalujaWouter L J Hinrichs
© 2021 Meta ULC. All rights reserved