Development of Bioink from Decellularized Tendon Extracellular Matrix for 3D Bioprinting

Macromolecular Bioscience
Burak ToprakhisarBahattin Koc

Abstract

Using decellularized extracellular matrix (dECM) hydrogels as bioinks has been an important step forward for bioprinting of functional tissue constructs, considering their rich microenvironment and their high degree of biomimicry. However, directly using dECM hydrogels as bioinks may not be suitable for bioprinting processes because of the loss of shape fidelity and geometrical precision of bioprinted structure due to their slow gelation kinetics. In this article, the development and direct bioprinting of dECM hydrogel bioink from bovine Achilles tendon were presented. The developed bioink is used for a microcapillary-based bioprinting process without any support structure and/or any additional cross-linker components. The reported decellularization and solubilization methods yield dECM pre-gels which turn into stable hydrogels in a short time at physiological conditions. The gelation kinetics and mechanical strength of bioinks with different concentrations and digestion times are characterized. A support structure-free 3D bioprinting of the developed bioink is shown by aspirating dECM bioinks and then in situ gelation and extrusion through a fine microcapillary nozzle. The viability assays indicate that the developed dECM bioi...Continue Reading

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Citations

Jun 7, 2019·Biofabrication·Amir K MiriAli Khademhosseini
Jul 22, 2020·Advanced Healthcare Materials·Alperen Abaci, Murat Guvendiren
May 5, 2020·Journal of Biomedical Materials Research. Part a·Kate Da SilvaViness Pillay
Apr 30, 2019·Small·Marcel Alexander HeinrichYu Shrike Zhang
Jul 1, 2020·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Jiadi LiangChang Liu
Oct 20, 2020·Advanced Materials Technologies·Elham DavoodiNureddin Ashammakhi
Dec 11, 2020·Advanced Materials·Martina M De SantisDarcy E Wagner
Mar 7, 2021·Bioengineering·Claire BenwoodStephanie M Willerth
Apr 4, 2021·Journal of Controlled Release : Official Journal of the Controlled Release Society·Sandra Ruiz-AlonsoJose Luis Pedraz
May 21, 2021·Frontiers in Cell and Developmental Biology·Marguerite MeeremansCatharina De Schauwer
Jun 8, 2021·Regenerative Therapy·Wenhui ZhangShenghua Chen
Jun 9, 2021·Biomedical Materials·Fatemeh HaghwerdiMohamadreza Baghaban Eslaminejad
Jul 1, 2021·Biofabrication·Susanna PilusoJennifer Patterson
Dec 23, 2021·Advanced Materials·Zeyu LuoYu Shrike Zhang

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