Development of biometric DNA ink for authentication security

The Tohoku Journal of Experimental Medicine
Masaki Hashiyada

Abstract

Among the various types of biometric personal identification systems, DNA provides the most reliable personal identification. It is intrinsically digital and unchangeable while the person is alive, and even after his/her death. Increasing the number of DNA loci examined can enhance the power of discrimination. This report describes the development of DNA ink, which contains synthetic DNA mixed with printing inks. Single-stranded DNA fragments encoding a personalized set of short tandem repeats (STR) were synthesized. The sequence was defined as follows. First, a decimal DNA personal identification (DNA-ID) was established based on the number of STRs in the locus. Next, this DNA-ID was encrypted using a binary, 160-bit algorithm, using a hashing function to protect privacy. Since this function is irreversible, no one can recover the original information from the encrypted code. Finally, the bit series generated above is transformed into base sequences, and double-stranded DNA fragments are amplified by the polymerase chain reaction (PCR) to protect against physical attacks. Synthesized DNA was detected successfully after samples printed in DNA ink were subjected to several resistance tests used to assess the stability of printin...Continue Reading

References

Jun 6, 2003·Forensic Science International : Synergy·M HashiyadaM Funayama

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Citations

Sep 18, 2012·PloS One·Ken Halvorsen, Wesley P Wong
Jun 17, 2009·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Jörg OehlmannCharles R Tyler
Feb 23, 2020·Angewandte Chemie·Robert N GrassWendelin J Stark
Jun 15, 2005·Analytical Chemistry·T A BrettellR Saferstein
Apr 12, 2021·International Journal of Pharmaceutics·Saad Asadullah ShariefEvangelyn Alocilja

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