Development of Bipotent Cardiac/Skeletal Myogenic Progenitors from MESP1+ Mesoderm

Stem Cell Reports
Sunny Sun-Kin ChanMichael Kyba

Abstract

The branchiomeric skeletal muscles co-evolved with new chambers of the heart to enable predatory feeding in chordates. These co-evolved tissues develop from a common population in anterior splanchnic mesoderm, referred to as cardiopharyngeal mesoderm (CPM). The regulation and development of CPM are poorly understood. We describe an embryonic stem cell-based system in which MESP1 drives a PDGFRA+ population with dual cardiac and skeletal muscle differentiation potential, and gene expression resembling CPM. Using this system, we investigate the regulation of these bipotent progenitors, and find that cardiac specification is governed by an antagonistic TGFβ-BMP axis, while skeletal muscle specification is enhanced by Rho kinase inhibition. We define transcriptional signatures of the first committed CPM-derived cardiac and skeletal myogenic progenitors, and discover surface markers to distinguish cardiac (PODXL+) from the skeletal muscle (CDH4+) CPM derivatives. These tools open an accessible window on this developmentally and evolutionarily important population.

References

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Citations

May 2, 2016·Biochemical and Biophysical Research Communications·Sunny Sun-Kin ChanMichael Kyba
May 18, 2016·The Journal of Cell Biology·Robert G Kelly
Sep 20, 2016·Journal of Cardiovascular Development and Disease·Heather Evans Anderson, Lionel Christiaen
Aug 25, 2016·Developmental Biology·Ashish R DeshwarIan C Scott
Feb 21, 2018·Acta Biochimica Et Biophysica Sinica·Jian WuJian Xu
Mar 15, 2019·EvoDevo·Maria Mandela PrünsterStefano Tiozzo
Apr 23, 2020·Anatomia, histologia, embryologia·Katrin BoraschJohanna Plendl
Jan 5, 2017·Cellular and Molecular Life Sciences : CMLS·Yu Liu
Jan 5, 2021·The Journal of Clinical Investigation·Anne-Claire GuénantinMichel Pucéat
Jan 9, 2021·Frontiers in Cell and Developmental Biology·Imadeldin YahyaBeate Brand-Saberi
Nov 8, 2020·Molecular Therapy : the Journal of the American Society of Gene Therapy·Jin-Woo LeeHyo-Soo Kim

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Datasets Mentioned

BETA
GSE74682

Methods Mentioned

BETA
fluorescence-activated cell sorting
RNA-seq
flow cytometry

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