Development of calcium signalling mechanisms during maturation of human oocytes

Molecular Human Reproduction
M HerbertA P Murdoch

Abstract

Sperm-induced Ca2+ signals mediate the events of oocyte activation at fertilization. In this study, the development of mechanisms involved in the generation of Ca2+ signals in human oocytes was investigated. The thiol reagent, thimerosal, which induces oscillations of intracellular Ca2+ ([Ca2+]i) similar to those seen during fertilization, was used to mobilize Ca2+ in in-vivo matured, immature and in-vitro matured human oocytes. There was an increase in the sensitivity to thimerosal during maturation of human oocytes, with oocytes from small antral follicles being relatively insensitive, compared with those from luteinized follicles, which displayed a large spike followed by sustained oscillations in [Ca2+]i. These oscillations were inhibited by caffeine which suggests that they were mediated by the inositol trisphosphate receptor Ca2+ release system. When immature oocytes were cultured in vitro they acquired the capacity to undergo a single large spike in [Ca2+]i, however, subsequent sustained oscillations were not observed, indicating that these oocytes failed to develop fully competent Ca2+ signalling mechanisms during culture in vitro. This finding may be a key factor in the poor developmental competence of in-vitro matured...Continue Reading

Citations

Feb 9, 2002·Molecular Reproduction and Development·Jorge A PiedrahitaH R Tervit
Feb 20, 2013·Journal of Assisted Reproduction and Genetics·Yi-Ran LiRi-Cheng Chian
Apr 25, 2003·The Journal of Steroid Biochemistry and Molecular Biology·M SchoenfelderR Einspanier
Mar 3, 2004·Reproductive Biomedicine Online·Ri-Cheng ChianSeang-Lin Tan
Oct 1, 2010·Zygote : the Biology of Gametes and Early Embryos·M T AjmatM I Bühler
Feb 4, 2012·Zygote : the Biology of Gametes and Early Embryos·M T AjmatM I Bühler
Feb 7, 2006·Reproductive Biology and Endocrinology : RB&E·Hai Feng WangTakato Terada
Jul 19, 2012·Zygote : the Biology of Gametes and Early Embryos·G Sánchez ToranzoM I Bühler
Apr 4, 2014·In Vitro Cellular & Developmental Biology. Animal·Karuppanan V Premkumar, Shail K Chaube
Oct 10, 2001·Human Fertility : Journal of the British Fertility Society·UNKNOWN Policy and Practice Sub-Committee, UNKNOWN Executive Committee of British Fertility Society
Jan 29, 2013·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Manuel MuñozMarcos Meseguer
Nov 6, 2008·Reproductive Biomedicine Online·Björn HeindryckxPetra De Sutter
Feb 4, 2006·Reproductive Biomedicine Online·Naiqing ChenSoon-Chye Ng
Mar 25, 2014·Reproductive Biomedicine Online·Frauke Vanden MeerschautPetra De Sutter
Jan 12, 2005·Current Topics in Developmental Biology·Geoffrey E Woodard, Juan A Rosado
Jul 11, 2007·Molecular Reproduction and Development·F MiyaraB Lefèvre
Jul 17, 2020·Open Biology·Paula SteinCarmen J Williams

❮ Previous
Next ❯

Related Concepts

Related Feeds

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.