Development of cetuximab-resistant human nasopharyngeal carcinoma cell lines and mechanisms of drug resistance

Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie
Qiang ZuoRong-cheng Luo

Abstract

This work aimed to explore the induction of cetuximab-resistant human nasopharyngeal carcinoma (hNPC) 5-8F/Erbitux cells and the mechanisms of drug resistance. The 5-8F cells with high EGFR expression and cetuximab sensitivity were screened and then induced by stepwise exposure to increasing doses of cetuximab. Western blot was conducted to detect protein levels. Our results are as follows: we successfully induced the cetuximab-resistant 5-8F/Erbitux hNPC cells. After treatment with cetuximab for 3 and 5 days, the RI was 1.2 and 1.1, respectively. The 5-8F/Erbitux cells showed cross-resistance to 5-FU (P<0.01) and some resistance to Taxol (P>0.05) as well as enhanced sensitivity to DDP (P>0.05). The cells had increased levels of P-gP, IGF-1R, P-IGF-1R, K-ras, H-ras, and PTEN protein expression (P<0.001), while survivin decreased (P<0.001). Through sequence alignments, gene mutations in the PTEN gene at exons 5, 7, and 8, as well as the H-ras and K-ras genes in codons 12, 13, 59, and 61, were not observed. After transfection with H-ras-shRNA plasmid, the 5-8F/Erbitux cells showed reduced levels of gene and protein expression of H-ras and elevated sensitivity to cetuximab. In conclusion, gene amplification and overexpression of H...Continue Reading

References

Sep 1, 1999·American Journal of Medical Genetics·B WeissK Shannon
Oct 8, 1999·São Paulo Medical Journal = Revista Paulista De Medicina·C M CoutinhoM A Nagai
Nov 27, 1999·Science·S Zimmermann, K Moelling
Mar 10, 2001·Experimental Cell Research·L Simpson, R Parsons
Jun 28, 2001·Oncogene·J Mendelsohn, J Baselga
Jul 12, 2002·Nature·Gregory J Hannon
Feb 18, 2004·International Journal of Radiation Oncology, Biology, Physics·K Kian AngLuka Milas
Mar 17, 2004·Cancer Treatment Reviews·Juan Angel Fresno VaraManuel González-Barón
Apr 6, 2005·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Anthony T C ChanAlex Y Chang
Feb 10, 2006·The New England Journal of Medicine·James A BonnerK Kian Ang
Apr 19, 2006·Cancer Research·Alex Toker, Merav Yoeli-Lerner
Jan 27, 2007·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Ingo K MellinghoffPaul S Mischel
Oct 3, 2007·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Chien-Hsing LuDihua Yu
Mar 14, 2008·The New England Journal of Medicine·Fortunato Ciardiello, Giampaolo Tortora
Sep 12, 2008·The New England Journal of Medicine·Jan B VermorkenRicardo Hitt
Oct 24, 2008·The New England Journal of Medicine·Christos S KarapetisJohn R Zalcberg
Jul 10, 2009·Annals of Oncology : Official Journal of the European Society for Medical Oncology·E Van CutsemUNKNOWN ESMO Guidelines Working Group
Jul 29, 2009·Nature Reviews. Clinical Oncology·Nicola NormannoFortunato Ciardiello
Aug 12, 2009·Current Drug Targets·F MeriggiA Zaniboni

❮ Previous
Next ❯

Citations

May 24, 2011·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Qiang ZuoWangjun Liao
Dec 23, 2011·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Qiang ZuoWangjun Liao
Jun 1, 2018·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Shilpa BhatiaSana D Karam
Mar 16, 2021·Frontiers in Oncology·Sandra Ortiz-CuaranPierre Saintigny

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Cell Cycle Pathways

Cell cycle is a complex process regulated by several signal transduction pathways and enzymes. Here is the latest research on regulation of cell cycle and cell cycle pathways.

Related Papers

The New England Journal of Medicine
Christos S KarapetisJohn R Zalcberg
Cell Research
Michael C LawrenceMelanie H Cobb
© 2021 Meta ULC. All rights reserved