Development of computer-assisted sperm analysis plugin for analyzing sperm motion in microfluidic environments using Image-J

Theriogenology
Mohamed ElsayedMohamed Abdelgawad

Abstract

We modified a previously reported computer-assisted sperm analysis (CASA) plugin for Image-J to enable analyzing motion of sperm cells in microfluidic environments. Microfluidics is increasingly being used in sperm-related applications such as sperm selection, IVF, and sperm motion behavior. Current CASA systems are not capable of analyzing motion of sperm cells in microfluidic devices where both sperm cells and the liquid itself are constantly moving, contrary to the conventional situation of sperm cells moving in a stationary liquid. We resolved this deficiency in the modified plugin reported here and built an image processing pipeline to enhance object detection, which increased CASA accuracy considerably. More importantly, particle tracking was improved and modified to accommodate sperm cells going out of focus for short periods during swimming on the same track. This last feature is particularly important in microfluidics where height of the microchannel is larger than that of CASA custom chambers to avoid channel blockage; this increased height causes sperm cells to frequently come in and out of focus. New parameters were introduced to allow studying new aspects of sperm motion behavior such as rheotaxis and wall tracking...Continue Reading

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Citations

May 18, 2017·Reproduction in Domestic Animals = Zuchthygiene·T M El-SherryM Abdelgawad
Nov 2, 2017·Nature Reviews. Urology·Reza NosratiDavid Sinton
Mar 14, 2018·Reproduction, Fertility, and Development·Marc YesteMaria M Rivera Del Álamo
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Jan 19, 2019·PLoS Computational Biology·Carlos Alquézar-BaetaRosaura Pérez-Pé
Feb 26, 2019·Biology of Reproduction·Denis A Vaughan, Denny Sakkas
Mar 4, 2020·Journal of Evolutionary Biology·Jessica H HadlowRowan A Lymbery
Jul 6, 2019·Biomedical Microdevices·A M EsmaeelM Abdelgawad
Nov 18, 2018·Scientific Reports·Jon Romero-AguirregomezcortaSean Fair
Dec 28, 2019·Reproduction in Domestic Animals = Zuchthygiene·Timor M El-SherryAhmed A Ezzat
May 18, 2020·Molecular Reproduction and Development·Kristin A Hook, Heidi S Fisher
Aug 12, 2020·Reproduction in Domestic Animals = Zuchthygiene·Mohammed A Abdel-GhaniMasashi Nagano
Aug 6, 2021·Environmental Science and Pollution Research International·Lucia De MarchiFabio Bulleri

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