Development of control material for hemoglobin analysis

Clinical Chemistry and Laboratory Medicine : CCLM
Sakorn PornprasertKallayanee Treesuwan

Abstract

Background: There is no certified control material for hemoglobin analysis which has the hemoglobin (Hb)A(2)/E level as high as found in ?-thalassemia trait, HbE trait, ?-thalassemia/HbE disease and homozygote of HbE, the thalassemia types found frequently in the Southeast Asian population. The aim of this study was to prepare the lyophilized hemoglobin control materials for hemoglobin analysis. Methods: Washed and dialysed erythrocytes of normal individuals and patients with ?-thalassemia trait, HbE trait, ?-thalassemia/HbE disease, homozygous HbE were lysed in 5% sucrose solution. The lyophilized hemoglobin control materials were prepared by using a freeze-drying (lyophilization) method. The high performance liquid chromatography (HPLC) analysis of lyophilized hemoglobin was performed after storing at ?20?C for 1, 15 and 30?days and for 3?months. Results: The chromatograms of lyophilized hemoglobin control materials showed similar patterns and similar levels of HbA, HbA(2)/E and HbF when compared with equivalent fresh whole blood. Moreover, the lyophilized hemoglobin presented a good correlation coefficient (r>0.990) of relationships between HPLC, low pressure liquid chromatography (LPLC) and capillary electrophoresis (CE) me...Continue Reading

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Citations

May 23, 2015·Clinical Chemistry and Laboratory Medicine : CCLM·Sakorn PornprasertKanokwan Jaiping
Oct 9, 2016·Clinical Chemistry and Laboratory Medicine : CCLM·Nattasit Pienthai, Sakorn Pornprasert

Related Concepts

High Pressure Liquid Chromatography Procedure
Liquid Chromatography
Erythrocytes
Fetal Hemoglobin
Freeze Drying
Hemoglobin A
Hemoglobin
Thalassemia
Electrophoresis, Capillary
South East Asian

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