Development of CpG-adjuvanted stable prefusion SARS-CoV-2 spike antigen as a subunit vaccine against COVID-19

Scientific Reports
Tsun-Yung KuoCharles Chen

Abstract

The COVID-19 pandemic is a worldwide health emergency which calls for an unprecedented race for vaccines and treatment. In developing a COVID-19 vaccine, we applied technology previously used for MERS-CoV to produce a prefusion-stabilized SARS-CoV-2 spike protein, S-2P. To enhance immunogenicity and mitigate the potential vaccine-induced immunopathology, CpG 1018, a Th1-biasing synthetic toll-like receptor 9 (TLR9) agonist was selected as an adjuvant candidate. S-2P in combination with CpG 1018 and aluminum hydroxide (alum) was found to be the most potent immunogen and induced high titer of neutralizing antibodies in sera of immunized mice against pseudotyped lentivirus reporter or live wild-type SARS-CoV-2. In addition, the antibodies elicited were able to cross-neutralize pseudovirus containing the spike protein of the D614G variant, indicating the potential for broad spectrum protection. A marked Th1 dominant response was noted from cytokines secreted by splenocytes of mice immunized with CpG 1018 and alum. No vaccine-related serious adverse effects were found in the dose-ranging study in rats administered single- or two-dose regimens of S-2P combined with CpG 1018 alone or CpG 1018 with alum. These data support continued de...Continue Reading

References

Apr 30, 2015·Immune Network·Sujin Lee, Minh Trang Nguyen
Oct 9, 2016·Methods in Molecular Biology·John D Campbell
Aug 16, 2017·Proceedings of the National Academy of Sciences of the United States of America·Jesper PallesenJason S McLellan
Feb 23, 2020·Science·Daniel WrappJason S McLellan
Feb 23, 2020·The Lancet Infectious Diseases·Ensheng DongLauren Gardner
Feb 29, 2020·The New England Journal of Medicine·Bill Gates
Mar 4, 2020·Nature Microbiology·UNKNOWN Coronaviridae Study Group of the International Committee on Taxonomy of Viruses
Apr 30, 2020·Nature Reviews. Immunology·Peter J HotezMaria Elena Bottazzi
Jun 9, 2020·BioRxiv : the Preprint Server for Biology·Wei LiDimiter S Dimitrov

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Citations

Dec 31, 2020·Vaccines·Shilu MathewHadi M Yassine
Jan 10, 2021·Advanced Drug Delivery Reviews·Jeroen PolletUlrich Strych
Jun 29, 2021·Expert Review of Vaccines·Chih-Cheng LaiPo-Ren Hsueh
Aug 21, 2021·Expert Review of Vaccines·Nan-Chang ChiuChien-Yu Lin
Aug 25, 2021·The Brazilian Journal of Infectious Diseases : an Official Publication of the Brazilian Society of Infectious Diseases·Mehrdad MohammadiHamed Mirzaei
Oct 12, 2021·Expert Review of Vaccines·Daniel MekonnenTengchuan Jin
Nov 6, 2021·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Chia-En LienCharles Chen

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Datasets Mentioned

BETA
MN908947
MT192759

Methods Mentioned

BETA
ELISA
glycosylation
transfection

Software Mentioned

GraphPad
Prism

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