Development of Cryptosporidium parvum-induced gastrointestinal neoplasia in severe combined immunodeficiency (SCID) mice: severity of lesions is correlated with infection intensity.

The American Journal of Tropical Medicine and Hygiene
Gabriela CertadE Dei-Cas

Abstract

We reported previously that Cryptosporidium parvum was able to induce intestinal tumors in severe combined immunodeficiency (SCID) mice treated with corticoids. To further characterize this Cryptosporidium-induced cell transformation, SCID mice treated with dexamethasone were challenged with C. parvum oocysts, and euthanatized sequentially after infection for histologic examination. Ki-67 was used as a marker of cellular proliferation. Our previous results were confirmed, and it was also found that mice receiving higher inocula (10(6)-10(7)) experienced more severe neoplastic development. Additionally, neoplastic changes were observed not only in the caecum but also in the stomach and duodenum of some animals. Interestingly, SCID mice (6/6) inoculated with 10(5)-10(7) oocysts showed high grade intraepithelial neoplasia or adenomas with high grade dysplasia in the caecum after Day 46 post-infection (PI). Immunohistochemistry for Ki-67 staining indicated the neoplastic process associated to cryptosporidiosis, and evidenced the first immunohistochemical alterations at early stages of the process, even at 3 weeks PI.

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Citations

Feb 22, 2012·Parasite : Journal De La Société Française De Parasitologie·S BenamrouzG Certad
Dec 14, 2011·AIDS Research and Human Retroviruses·Fatma M SheblJames J Goedert
Jul 6, 2012·Clinical Microbiology Reviews·Stephanie M FletcherJohn Ellis
Mar 22, 2014·Disease Models & Mechanisms·Sadia BenamrouzGabriela Certad
Jul 22, 2014·The Journal of Eukaryotic Microbiology·Cécile-Marie Aliouat-Denis
Apr 20, 2018·PloS One·Violetta Sulżyc-BielickaWojciech Rogowski
May 2, 2020·Scientific Reports·Christophe AudebertMagali Chabé
Aug 31, 2019·Cancer Investigation·Sidhant JainPooja Jain
Oct 31, 2020·Microorganisms·Manasi SawantSadia Benamrouz-Vanneste

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