Development of desiccation tolerance and vitrification by preculture treatment in suspension-cultured cells of the liverwort Marchantia polymorpha

Planta
Rie Hatanaka, Yasutake Sugawara

Abstract

Some cultured plant cells are able to acquire tolerance to various stresses when they are cultured under suitably controlled conditions. Induction of a high level of desiccation tolerance in suspension-cultured cells of the liverwort Marchantia polymorpha was examined for studying the mechanisms of desiccation tolerance and vitrification at the cellular level. Desiccation tolerance level of cells was very low and the survival rate was less than 10% after exposure to drying below 0.1 g H(2)O g(-1) dry weight (DW). Preculture treatment in 0.5 M sucrose medium was the most effective method for inducing a high level of desiccation tolerance in cells and the survival rate was 87% even after being desiccated to below 0.1 g H(2)O g(-1) DW. Preculture treatment caused alteration of cell structures and accumulation of a large amount of sucrose and newly synthesized proteins in cells. Abundant sucrose and preculture-induced proteins were necessary for full development of desiccation tolerance in the cells. When water content decreased to below 0.1 g H(2)O g(-1) DW, desiccation-tolerant cells that had been precultured were vitrified above 0 degrees C and maintained stable viability. We have succeeded in the induction of desiccation tolera...Continue Reading

References

Jan 1, 1992·Annual Review of Physiology·J H CroweL M Crowe
Jun 9, 1988·Biochimica Et Biophysica Acta·J H CroweT J Anchordoguy
Mar 25, 1972·Lancet·A G BaikieJ N Lickiss
Apr 1, 1996·Biophysical Journal·W Q SunJ H Crowe
Apr 29, 1998·Annual Review of Physiology·J H CroweL M Crowe
May 9, 2001·Biochimica Et Biophysica Acta·W F WolkersF A Hoekstra
Sep 7, 2001·Trends in Plant Science·F A HoekstraJ Buitink
Feb 16, 2002·Cryobiology·A E OliverJ H Crowe
Jan 20, 2004·Trends in Plant Science·Michael J Wise, Alan Tunnacliffe
Jun 1, 1996·Annual Review of Plant Physiology and Plant Molecular Biology·J. Ingram, D. Bartels
May 26, 2004·Cryobiology·Julia Buitink, Olivier Leprince
Jan 6, 2005·The Biochemical Journal·Kshamata GoyalAlan Tunnacliffe
Feb 8, 2005·Plant & Cell Physiology·Ed EtxeberriaJavier Pozueta-Romero
Sep 1, 1983·Plant Physiology·T H Chen, L V Gusta
Mar 1, 1989·Plant Physiology·R J Williams, A C Leopold
May 1, 1991·Plant Physiology·K L Koster
Jun 1, 1991·Plant Physiology·F Bruni, A C Leopold
Jun 13, 2006·The Australian Journal of Physiotherapy·Karl Schurr, Louise Ada
Feb 20, 2007·Biochimica Et Biophysica Acta·Thomas LennéKaren L Koster
Nov 1, 2005·Integrative and Comparative Biology·Melvin J OliverBrent D Mishler

❮ Previous
Next ❯

Citations

Mar 19, 2011·Journal of Plant Research·Daisuke TakezawaYoichi Sakata
Aug 25, 2018·Scientific Reports·Luis D AlcarazMario A Arteaga-Vázquez
Aug 6, 2020·Frontiers in Plant Science·Yufei SunAssaf Mosquna
Nov 30, 2020·American Journal of Botany·Kirsten K CoeLloyd R Stark

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.