Development of dual-compartment perfusion bioreactor for serial coculture of hepatocytes and stellate cells in poly(lactic-co-glycolic acid)-collagen scaffolds

Journal of Biomedical Materials Research. Part B, Applied Biomaterials
F WenH Yu

Abstract

An important challenge in liver tissue engineering is to overcome the rapid loss of hepatocyte functions. In vivo, hepatocytes are compact polyhedral cells with round nuclei; however, they readily loss many of their differentiated functions in vitro. To overcome this challenge, we have established a new perfusion bioreactor that consists of two compartments which enabled the serial coculture of hepatocytes and hepatic stellate cells-T6 without direct contact between each other. Three dimensional scaffolds were utilized in the bioreactor as physical anchors for cells. The scaffolds consist of collagen grafted poly(lactic-co-glycolic acid) microfibers and cross-linked collagen sponges between microfibers for additional cellular support and adhesion. The advantages of this new bioreactor are enabling cell culture in three dimensional organization and controlling the culture parameters of the supporting cells independently from the hepatocytes. The results showed that the hepatocytes exhibited much higher levels of the differentiated functions such as albumin secretion, urea synthesis, and cytochrome P450 enzymatic activity when compared with the monoculture system where hepatocytes alone were cultured. This perfusion bioreactor sy...Continue Reading

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Citations

Jun 18, 2011·Frontiers of Medicine·Jinzheng LiJianping Gong
Jun 20, 2014·World Journal of Gastroenterology : WJG·Cheng-Bo YuLan-Juan Li
Jul 25, 2014·Journal of Materials Science. Materials in Medicine·Shichang ZhangYingjie Wang
Oct 20, 2009·Current Opinion in Biotechnology·Janet W C Kung, Stuart J Forbes
Sep 27, 2012·Biophysical Journal·Lakshmi VenkatramanHanry Yu
Jan 3, 2016·Biotechnology and Bioengineering·Bao-Ngoc B NguyenJohn P Fisher
Jul 20, 2011·Journal of Polymer Science. Part B, Polymer Physics·Bret D UleryCato T Laurencin
Feb 10, 2018·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Janice C Y LiaoAlphonsus K S Chong
Jun 7, 2015·Archives of Toxicology·Sara Crespo YanguasMathieu Vinken
Apr 9, 2010·Journal of the Royal Society, Interface·J M BloomfieldG Landini
Jan 1, 2010·Archives of Histology and Cytology·Akina ChibaShigeki Hirano

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