Development of efficient protocol for rice transformation overexpressing MAP kinase and their effect on root phenotypic traits

Protoplasma
Pallavi SinghAlok Krishna Sinha

Abstract

Exhaustive studies on mitogen-activated protein kinase (MAPK) have reported the importance in regulating a variety of responses during plant growth and development. In particular, the potential MAPK genes, MPK3 and MPK6, seem to regulate a plethora of responses, conferring tolerance to varied abiotic, biotic, and developmental stimuli. This makes both MPK3 and MPK6 potential targets for further studies. It would be an important concern to overexpress and knock out these pivotal proteins and then, in turn, to monitor the plant response which is expected to correlate action of a gene to a trait in cellular and organismal contexts. However, overexpression of MAPK genes has remained a puzzle in plants. In the present study, we report the generation of stable transgenic lines overexpressing OsMPK3 in indica and japonica cultivars and OsMPK6 in japonica cultivar under the control of an inducible promoter. We also establish the crucial steps and troubleshooting for each of the indicated rice transformation medium components. Later, we study the potential role of these MAPKs in high-throughput analysis of root system architectural (RSA) traits. It was observed that OsMPK6 overexpression lines had a more robust and spread out root archi...Continue Reading

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Citations

Dec 12, 2021·Critical Reviews in Microbiology·Hajar YaakoubNicolas Papon

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Methods Mentioned

BETA
environmental stresses
transgenic
environmental stress
transgenics
phosphotransferase
PCR
RSA

Software Mentioned

GiA Roots

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