PMID: 7518698Apr 1, 1994Paper

Development of enzyme immunoassays specific for keratan sulphate- and core-protein-epitopes of the large aggregating proteoglycan from human articular cartilage

European Journal of Clinical Chemistry and Clinical Biochemistry : Journal of the Forum of European Clinical Chemistry Societies
D C FischerH D Haubeck

Abstract

In the course of chronic inflammatory and degenerative joint diseases proteoglycans are degraded by the action of proteases and oxygen radicals. Therefore, proteoglycan fragments, released from cartilage into the peripheral blood, might be useful markers of cartilage degradation. Sensitive enzyme immunoassays are useful for the detection of these proteoglycan fragments in serum. We therefore developed specific monoclonal antibodies against the large aggregating proteoglycan (aggrecan), which has been isolated and purified from human articular cartilage. Two monoclonal antibodies which recognize a novel cartilage-specific epitope on the keratan sulphate chain of aggrecan (mAb 4B3/D10) and an epitope of the core-protein of aggrecan (4G4/A10) were selected for the development of competitive enzyme-immunoassays. These assays allow the sensitive and specific detection of cartilage-derived proteoglycan fragments, not only in synovial fluid but also in serum. They can now be used for the study of inflammatory and degenerative joint diseases.

References

Nov 15, 1981·Analytical Biochemistry·A S EdgeP Weber

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Citations

Oct 27, 2009·Best Practice & Research. Clinical Rheumatology·Berent J Prakken, Salvatore Albani
Dec 23, 2011·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Partha S NagchowdhuriAnthony A Capehart
Dec 10, 2014·Anatomia, histologia, embryologia·M Kobayashi-MiuraY Fujita
May 7, 2014·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Cheng ZhouJames A Martin
Dec 25, 2010·Birth Defects Research. Part C, Embryo Today : Reviews·Cosimo De BariAndrea Augello
Nov 26, 2013·Journal of Anatomy·Niamh C Nowlan, James Sharpe

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