Jun 1, 1996

Development of enzyme-linked immunoassays for human angiotensin I converting enzyme suitable for large-scale studies

Journal of Hypertension
S M DanilovF Alhenc-Gelas

Abstract

To develop and validate a simple immunological assay for human angiotensin converting enzyme (ACE) based on monoclonal antibodies. Microtitre plates were coated with mouse monoclonal antibody (MoAb) to human ACE (9B9) and incubated with diluted samples of human plasma. In the sandwich enzyme-linked immunosorbent assay (ELISA), the plasma ACE, bound to MoAb 9B9, was revealed using polyclonal anti-ACE antibodies and alkaline phosphatase conjugated to goat anti-rabbit immunoglobulin G. In the plate precipitation assay the ACE activity, quantitatively precipitated from human plasma by MoAb 9B9, was measured by enzymatic fluorimetric assay with p-benzyloxycarboxyl-glycyl-L-histidyl-L-leucine or p-benzyloxycarboxyl-L-phenylalanyl-L-histidyl-L-leucine as substrate, directly in the wells. These assays are specific for the amino-terminal domain of ACE and recognize differences in the conformations of native and recombinant ACE. The sensitivity of the sandwich ELISA was 200 pg/ml assay medium; it quantifies the ACE in 10 microliters human plasma or less. Intra- and inter-assay variability coefficients were 6.2 and 13.6%, respectively. Both variants of the assay determined the plasma ACE concentration in the presence of ACE inhibitors or ...Continue Reading

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Mentioned in this Paper

Human plasma
Monoclonal Antibodies
Monoclonal antibodies, antineoplastic
Alkaline Phosphatase Measurement
Gene Deletion Abnormality
Gene Deletion
Alkaline Phosphatase
Angiotensin Converting Enzyme Measurement
Angiotensin Converting Enzyme Activity
Enzyme Assays

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