Development of Enzyme Loaded Polyion Complex Vesicle (PICsome): Thermal Stability of Enzyme in PICsome Compartment and Effect of Coencapsulation of Dextran on Enzyme Activity

Macromolecular Bioscience
Hengmin TangAkihiro Kishimura

Abstract

Applications of enzymes are intensively studied, particularly for biomedical applications. However, encapsulation or immobilization of enzymes without deactivation and long-term use of enzymes are still at issue. This study focuses on the polymeric vesicles "PICsomes" for encapsulation of enzymes to develop a hecto-nanometer-scaled enzyme-loaded reactor. The catalytic activity of a PICsome-based enzyme nanoreactor is carefully examined to clarify the effect of compartmentalization by PICsome. Encapsulation by PICsome provides a stability enhancement of enzymes after 24 h incubation at 37 °C, which is particularly helpful for maintaining the high effective concentration of β-galactosidase. Moreover, to control the microenvironment inside the nanoreactor, a large amount of dextran, a neutral macromolecule, is encapsulated together with β-galactosidase in the PICsome. The resulting dextran-coloaded nanoreactor contributes to the enhancement of enzyme stability, even after exposure to 24 h incubation at -20 °C, mainly due to the antifreezing effect.

References

Sep 1, 1982·Cell·A B Fulton
Oct 9, 2001·Trends in Biochemical Sciences·R J Ellis
Jun 24, 2003·Cryobiology·Zdenek Hubálek
Jan 27, 2004·Journal of Molecular Biology·Dimos BolisPiero Andrea Temussi
Jan 18, 2005·Journal of Controlled Release : Official Journal of the Controlled Release Society·Gerard HuysmansPatrick Van Gelder
Jun 14, 2005·Comptes rendus biologies·Brian W Matthews
Apr 17, 2007·Trends in Biotechnology·Robert EisenthalDavid W Hough
Jan 29, 2008·Trends in Biotechnology·Eduardo A CeccarelliOscar A Roveri
Mar 14, 2009·Small·Qi ChenG Julius Vancso
Jan 19, 2010·Journal of the American Chemical Society·Yasutaka AnrakuKazunori Kataoka
Jul 5, 2012·PloS One·Tobias Vöpel, George I Makhatadze
Oct 6, 2012·Advanced Drug Delivery Reviews·Theresa M Allen, Pieter R Cullis
Jun 1, 2013·Biomacromolecules·Iria Louzao, Jan C M van Hest
Dec 5, 2013·Biophysical Chemistry·Isabel PastorFrancesc Mas

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Citations

Mar 25, 2018·Chemistry : a European Journal·Jean Felix MukerabigwiKazunori Kataoka
Mar 28, 2019·Soft Matter·Whitney C Blocher McTigue, Sarah L Perry
May 5, 2020·Small·Whitney C Blocher McTigue, Sarah L Perry
Apr 4, 2021·Macromolecular Bioscience·Xin LiXikuang Yao
May 7, 2021·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Akihiro Kishimura
Oct 12, 2021·Biomacromolecules·Ying CaiJunyou Wang

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