PMID: 6166351Jan 5, 1981Paper

Development of ganglion cells and their axons in organized cultures of fetal mouse retinal explants

Brain Research
N R SmalheiserM B Bornstein

Abstract

Retinas from 13-15 day fetal mice were explanted alone, with adjacent eyeball tissue, or with nearby superior colliculus explants. The organotypic structure of the retina developed in situ, including photoreceptors, interneurons, plexiform layers, ganglion cells, and an optic fibre layer. Electrophysiologic recordings demonstrated that functional synaptic networks developed resembling bioelectric response patterns seen in situ. Within half-retinas, arrays of optic fibers converged to the optic nerve head; in co-cultures with tectum they could become myelinated. Large bundles of long, naked neurites--1 degree primary retinal fibers--emerged from the explant in the first few days in vitro; these could often be traced back to the optic nerve head and a detailed survey of their properties using horseradish peroxidase (HRP) tracing methods identified tham as ganglion cell axons. When growing upon collagen substrata, 1 degree fibers began to disintegrate during the second week in vitro; however, many 1 degree fibers that grew into superior colliculus explants were maintained for at least 5 weeks in vitro, where they formed elaborate, functional terminal arborizations. In a few cases, 1 degree fibers grew across skeletal muscle fibers...Continue Reading

References

Nov 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·D G PuroM Nirenberg
May 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·R R RuffoloM Nirenberg
Dec 7, 1978·Nature·L Ziskind, M J Dennis
Dec 17, 1976·Brain Research·G E Landreth, B W Agranoff
May 1, 1975·Developmental Biology·P C Letourneau
Oct 1, 1975·The Journal of Comparative Neurology·W Franklin Hughes, A La Velle
Sep 1, 1972·The Journal of Cell Biology·T Elsdale, J Bard
Mar 22, 1974·Annals of the New York Academy of Sciences·S M Crain, E R Peterson
Feb 1, 1973·Experimental Cell Research·E B Masurovsky, E R Peterson
Apr 1, 1974·The Journal of Cell Biology·P C Letourneau, N K Wessells
Jan 1, 1971·Zeitschrift für Zellforschung und mikroskopische Anatomie·M M La Vail, W Hild
Sep 1, 1970·The Anatomical Record·Y F Pei, J A Rhodin
Jan 1, 1967·Zeitschrift für Zellforschung und mikroskopische Anatomie·W Hild, G Callas
Oct 1, 1963·Journal of Neurology, Neurosurgery, and Psychiatry·J M VANBUREN

❮ Previous
Next ❯

Citations

Dec 1, 1982·Journal of Neurocytology·P M RichardsonS Shemie
Apr 1, 1986·Developmental Biology·P Grant, Y Tseng
Jan 1, 1984·Brain Research·N R SmalheiserL M Reid
Aug 20, 1993·Brain Research. Developmental Brain Research·K R HuxlinM R Bennett
Jul 27, 2001·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·N K DhingraY Ramamohan
Aug 28, 1999·The Journal of Comparative Neurology·M M Tan, A R Harvey
Dec 19, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Nathalie Escande-BeillardDaniel L Kaufman
Mar 22, 2018·Oxidative Medicine and Cellular Longevity·Adrian SmedowskiJoanna Lewin-Kowalik
Aug 31, 2019·Investigative Ophthalmology & Visual Science·Kimberly K GokoffskiMin Zhao

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.