Development of gelatin-chitosan-hydroxyapatite based bioactive bone scaffold with controlled pore size and mechanical strength

Journal of Biomaterials Science. Polymer Edition
Kanchan MajiAkalabya Bissoyi

Abstract

Hydroxyapatite-chitosan/gelatin (HA:Chi:Gel) nanocomposite scaffold has potential to serve as a template matrix to regenerate extra cellular matrix of human bone. Scaffolds with varying composition of hydroxyapatite, chitosan, and gelatin were prepared using lyophilization technique where glutaraldehyde (GTA) acted as a cross-linking agent for biopolymers. First, phase pure hydroxyapatite-chitosan nanocrystals were in situ synthesized by coprecipitation method using a solution of 2% acetic acid dissolved chitosan and aqueous solution of calcium nitrate tetrahydrate [Ca(NO3)2,4H2O] and diammonium hydrogen phosphate [(NH4)2H PO4]. Keeping solid loading constant at 30 wt% and changing the composition of the original slurry of gelatin, HA-chitosan allowed control of the pore size, its distribution, and mechanical properties of the scaffolds. Microstructural investigation by scanning electron microscopy revealed the formation of a well interconnected porous scaffold with a pore size in the range of 35-150 μm. The HA granules were uniformly dispersed in the gelatin-chitosan network. An optimal composition in terms of pore size and mechanical properties was obtained from the scaffold with an HA:Chi:Gel ratio of 21:49:30. The composite...Continue Reading

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Citations

Feb 18, 2016·International Journal of Biomaterials·Kanchan MajiAkalabya Bissoyi
Jun 27, 2017·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Niranjan RameshGeorge J Dias
Aug 18, 2016·Organic & Biomolecular Chemistry·Jing HuJian Yin
Aug 23, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Alicia AguilarGuoqiang Hua
May 22, 2020·Biomedical Materials·Kanchan MajiMukesh Kumar Gupta
Sep 19, 2019·Journal of Biomaterials Science. Polymer Edition·Yogendra Pratap SinghRakesh Bhaskar
Oct 14, 2020·Stem Cells Translational Medicine·Swati MidhaSujata Mohanty
Sep 9, 2019·International Journal of Biological Macromolecules·Somayeh Vandghanooni, Morteza Eskandani

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Methods Mentioned

BETA
dynamic light scattering
scanning electron microscopy
ELISA

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