Development of gold nanoparticles biosensor for ultrasensitive diagnosis of foot and mouth disease virus

Journal of Nanobiotechnology
Mervat E HamdyDario Compagnone

Abstract

Nano-PCR is a recent tool that is used in viral diseases diagnosis. The technique depends on the fundamental effects of gold nanoparticles (AuNPs) and is considered a very effective and sensitive tool in the diagnosis of different diseases including viral diseases. Although several techniques are currently available to diagnose foot and mouth disease virus (FMDV), a highly sensitive, highly specific technique is needed for specific diagnosis of the disease. In the present work, a novel AuNPs biosensor has been designed using thiol-linked oligonucleotides that recognize the conserved 3D gene of FMDV. The AuNPs-FMDV biosensor specifically recognizes RNA standards of FMDV, but not that of swine vesicular disease virus (SVDV) isolates. The analytical sensitivity of the AuNPs-FMDV biosensor was 10 copy number RNA standards in RT-PCR and 1 copy number RNA standard in real-time rRT-PCR with a 94.5% efficiency, 0.989 R2, a - 3.544 slope and 100% specificity (no cross-reactivity with SVDV). These findings were confirmed by the specific and sensitive recognition of 31 Egyptian FMDV clinical isolates that represents the three FMDV serotypes (O, A, and SAT2). The AuNPs-FMDV biosensor presents in this study demonstrates a superior analytica...Continue Reading

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Citations

Nov 13, 2019·Artificial Cells, Nanomedicine, and Biotechnology·Wen LiYuanyan Liu
Mar 27, 2020·Current Topics in Medicinal Chemistry·Prasad MinakshiGaya Prasad
Sep 15, 2020·Journal of Nanoparticle Research : an Interdisciplinary Forum for Nanoscale Science and Technology·Iago Dillion Lima Cavalcanti, Mariane Cajubá de Britto Lira Nogueira
Dec 10, 2020·Metallomics : Integrated Biometal Science·Jemmyson Romário de Jesus, Tatianny de Araújo Andrade
Dec 29, 2020·Biotechnology and Applied Biochemistry·Jing LiHuitao Zhang
Nov 17, 2020·Frontiers in Microbiology·Sushmita PatraJyoti Ranjan Rout
Mar 10, 2021·Sustainable Chemistry and Pharmacy·Sepideh AhmadiMichael R Hamblin
May 29, 2021·Analytical and Bioanalytical Chemistry·Nadja E Solis-MarcanoCarlos R Cabrera
Sep 4, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Ilyas OzcicekAsel Aydeger
Sep 12, 2020·Pharmaceutical Nanotechnology·Sevda Şenel

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Methods Mentioned

BETA
PCR
biosensors
quartz crystal microbalance
biosensor
surface-enhanced Raman scattering
Dynamic light scattering
electron
transmission electron microscopy
electrophoresis

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