Development of hapten-linked microimmunoassays on polycarbonate discs

Analytical Chemistry
Jesús Tamarit-LópezAngel Maquieira

Abstract

An amino-modified polycarbonate surface of compact discs is used to link haptens covalently and directly as an alternative to the classic protein-hapten conjugate adsorption coating strategy employed in immunoassays. The modified surface maintains its physical and optical properties, and a standard disk drive can then read the assay results. Advantages are evaluated, such as the use of a broader spectrum of coupling media including organic solvents that are inappropriate for proteins but necessary for some water-insoluble haptens and the bypassing of the synthesis and purification for protein conjugates. As proof of concept, competitive microimmunoassays were developed for chlorpyrifos, atrazine, and 2-(2,4,5-trichlorophenoxy)propionic acid (2,4,5-TP), in microarray format, obtaining detection limits of 37.2, 8.1, and 76 ng/L, respectively. The sensitivity was 1 order of magnitude better than that obtained for all the studied systems using hapten-protein conjugates adsorbed on polystyrene enzyme-linked immunosorbent assay (ELISA) plates and polycarbonate surfaces. Further, the influence of hapten structure and presentation on molecular recognition pattern is discussed. To our knowledge, this is the first time that microarray an...Continue Reading

References

Feb 26, 1993·Journal of Immunological Methods·A NiveleauR Dante
Mar 21, 1998·Journal of Immunological Methods·O HofstetterB S Green
Sep 27, 2002·Analytical and Bioanalytical Chemistry·Sergi MoraisAngel Maquieira
Oct 7, 2003·Organic & Biomolecular Chemistry·James J La Clair, Michael D Burkart
May 12, 2005·Environmental Science & Technology·Eva M BrunAngel Maquieira
May 12, 2005·Journal of Molecular Biology·Edwin PozharskiDagmar Ringe
Nov 24, 2005·Journal of Agricultural and Food Chemistry·Eva M BrunAngel Maquieira
Aug 4, 2006·Organic & Biomolecular Chemistry·James J La Clair, Michael D Burkart
Jan 18, 2007·Chembiochem : a European Journal of Chemical Biology·Olivier CarionOleg Melnyk
Sep 15, 2007·Analytical Chemistry·Sergi MoraisAngel Maquieira
Sep 20, 2007·Bioconjugate Chemistry·María-José BañulsAngel Maquieira
Jan 18, 2008·Biophysical Journal·Hyunsook JungPaul S Cremer
Feb 5, 2008·Analytica Chimica Acta·Jesús Tamarit-LópezAngel Maquieira
Feb 8, 2008·Bioconjugate Chemistry·María-José BañulsAngel Maquieira
Jul 4, 2008·Analytical and Bioanalytical Chemistry·Sergi MoraisAngel Maquieira
Jun 16, 2009·Analytical Chemistry·Sergi MoraisAngel Maquieira

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Citations

Oct 3, 2012·Accounts of Chemical Research·Hua-Zhong YuLily M-L Ou
Feb 29, 2016·Analytical and Bioanalytical Chemistry·Sergi MoraisÁngel Maquieira
Mar 25, 2014·The Analyst·Christine F Woolley, Mark A Hayes
Mar 16, 2013·Analytical Chemistry·Eva M BrunÁngel Maquieira
Nov 5, 2010·Environmental Science & Technology·Sergi MoraisAngel Maquieira
Feb 20, 2015·ACS Applied Materials & Interfaces·Virginia VanDelinderGeorge D Bachand
Aug 11, 2018·ACS Applied Materials & Interfaces·Ellen P A van HeeswijkAlbertus P H J Schenning

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