Development of hemoglobin typing control materials for laboratory investigation of thalassemia and hemoglobinopathies

Clinical Chemistry and Laboratory Medicine : CCLM
Sakorn PornprasertKanokwan Jaiping

Abstract

To date, the hemoglobin (Hb) typing control materials for laboratory investigation of thalassemia with low (1.8%-3.2%) and high (4%-6%) levels of HbA2 are available but there are no Hb typing quality control materials for analysis of thalassemia and hemoglobinopathies which are highly prevalent in South-East Asian countries. The main aim of the present study was to develop the lyophilized Hb typing control materials for laboratory investigation of thalassemia and hemoglobinopathies that are commonly found in South-East Asia. Erythrocytes of blood samples containing Hb Bart's, HbH, HbE, HbF, Hb Constant Spring (CS), Hb Hope, and Hb Q-Thailand were washed and dialysed with 0.85% saline solution. The erythrocytes were then lysed in 5% sucrose solution. The lyophilized Hb typing control materials were prepared by using a freeze drying (lyophilization) method. The high performance liquid chromatography (HPLC) analysis of lyophilized Hb was performed after the storage at -20 °C for 1 year and also after reconstitution and storage at 4 or -20 °C for 30 days. In addition, the Hb analysis was compared between the three different methods of HPLC, low pressure liquid chromatography (LPLC) and capillary electrophoresis (CE). Following a ye...Continue Reading

References

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Citations

Sep 10, 2015·Clinical Chemistry and Laboratory Medicine : CCLM·Giuseppe Lippi, Mario Plebani
Sep 24, 2015·Clinical Chemistry and Laboratory Medicine : CCLM·Sakorn Pornprasert
Dec 10, 2019·Clinical Chemistry and Laboratory Medicine : CCLM·Nattasit PienthaiSakorn Pornprasert
Feb 9, 2021·Clinical Chemistry and Laboratory Medicine : CCLM·Paweena YanpanyaSakorn Pornprasert

Methods Mentioned

BETA
PCR
electrophoresis

Related Concepts

Blood Preservation
High Pressure Liquid Chromatography Procedure
Erythrocytes
Hemoglobinopathies
Eryhem
Thalassemia
Electrophoresis, Capillary
High Pressure Liquid Chromatography Procedure
Liquid Chromatography
Erythrocytes

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