Development of high amylose wheat through TILLING.

BMC Plant Biology
Ann J SladeVic C Knauf

Abstract

Wheat (Triticum spp.) is an important source of food worldwide and the focus of considerable efforts to identify new combinations of genetic diversity for crop improvement. In particular, wheat starch composition is a major target for changes that could benefit human health. Starches with increased levels of amylose are of interest because of the correlation between higher amylose content and elevated levels of resistant starch, which has been shown to have beneficial effects on health for combating obesity and diabetes. TILLING (Targeting Induced Local Lesions in Genomes) is a means to identify novel genetic variation without the need for direct selection of phenotypes. Using TILLING to identify novel genetic variation in each of the A and B genomes in tetraploid durum wheat and the A, B and D genomes in hexaploid bread wheat, we have identified mutations in the form of single nucleotide polymorphisms (SNPs) in starch branching enzyme IIa genes (SBEIIa). Combining these new alleles of SBEIIa through breeding resulted in the development of high amylose durum and bread wheat varieties containing 47-55% amylose and having elevated resistant starch levels compared to wild-type wheat. High amylose lines also had reduced expression ...Continue Reading

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Datasets Mentioned

BETA
AF338431
FM865435

Methods Mentioned

BETA
transgenic
PCR
light microscopy
targeted mutation
genotyping
Assay

Software Mentioned

Scaffold
GraphPad
GPM manager
GPM extreme edition
PARSESNP
SDS
TILLING
GraphPad InStat
Adobe Photoshop
Proteome

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